Fluorescent and colorimetric detection of pH by a rhodamine-based probe
Fluorescent and colorimetric detection of pH by a rhodamine-based probe
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DOI：
10.1016/j.snb.2013.12.023
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发表时间：
2014-04-01
影响因子：
8.4
通讯作者：
Zhang, Wenqin
中科院分区：
文献类型：
--
作者：
Liu, Lijuan;Guo, Ping;Zhang, Wenqin
这里描述的是一种基于罗丹明的衍生物 (1)，它充当比色和荧光“开关”探针，用于检测 pH 值。探针的设计策略基于罗丹明染料的螺环（非荧光）和醌型（荧光）形式之间的结构变化。 1的四氢呋喃/三(羟甲基)氨基甲烷-HCl(4:6，v:v，pH 7.5)缓冲溶液是无色且无荧光的，而相应的螺内酰胺在H+诱导下开环成共轭醌形式，产生强烈的吸收和荧光发射以及粉红色。 1的荧光变化是可逆的，主要发生在pH 1.1~4.0范围内，对于强酸性环境的检测具有重要价值。皇冠版权所有 (C) 2013 由 Elsevier B.V. 出版。保留所有权利。
Described here is a rhodamine-based derivative (1) that acts as a colorimetric and fluorescent "off-on" probe for the detection of pH. The design tactics for the probe is based on the change in structure between spirocyclic (non-fluorescent) and quinoid-type (fluorescent) forms of rhodamine dyes. The tetrahydrofuran/Tris(hydroxymethyl)aminomethane-HCl (4:6, v:v, pH 7.5) buffer solution of 1 is colorless and nonfluorescent, whereas ring-opening of the corresponding spirolactam to a conjugated quinoid form induced by H+ gives rise to strong absorption and fluorescence emission as well as a pink color. The fluorescence change of 1 is reversible and takes place mainly within the pH range from 1.1 to 4.0, which is valuable for the detection of strong acidic environment. Crown Copyright (C) 2013 Published by Elsevier B.V. All rights reserved.