Immunization with the N-terminal region of the nonstructural protein NS1 promotes survival after challenge with lethal influenza A virus dose.
Immunization with the N-terminal region of the nonstructural protein NS1 promotes survival after challenge with lethal influenza A virus dose.
复制标题
使用非结构蛋白 NS1 的 N 末端区域进行免疫可促进致命甲型流感病毒攻击后的存活率。
DOI：
10.1089/vim.1998.11.131
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Ennis,FA
中科院分区：
文献类型：
--
作者：
Tamura,M;Saikh,KU;Kurane,I;Ennis,FA
We previously reported that the epitope recognized by an influenza A virus Hl, H2, and H3-crossreactive, H-2 Ld-restricted CD8+cytotoxic T lymphocyte (CTL) is located between amino acids 1 and 40 on the nonstructural protein NS1. In the present experiments, we examined whether immunization with recombinant vaccinia virus which contained genes coding for amino acids 1–40 of NS1 (Vac-10) protected mice from lethal challenge with influenza A virus. Mice immunized with this recombinant virus developed influenza A virus-specific cytotoxic activity but not neutralizing antibodies. Challenge with a lethal dose of influenza A virus demonstrated that the first deaths were delayed by 2 days, and the mortality rate was significantly reduced (p< 0.05) in Vac-10-immunized mice compared with mice immunized with control vaccinia virus. These results suggest that immunization with a single subtype-crossreactive CTL epitope on NS1 can induce protective immunity against lethal influenza A virus infection.
