Effect of different anesthesia methods on erythrocyte immune function in mice
Effect of different anesthesia methods on erythrocyte immune function in mice
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DOI：
10.1016/s1995-7645(13)60179-0
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发表时间：
2013-12-01
影响因子：
3.1
通讯作者：
Duan, Chen-Yang
中科院分区：
文献类型：
--
作者：
Zhang, Wen-Long;Liu, Meng-Ying;Duan, Chen-Yang
目的：探讨不同麻醉方法、不同麻醉药物对小鼠红细胞免疫功能的影响。方法：采用异氟烷、乙醚吸入麻醉，戊巴比妥钠、水合氯醛腹腔麻醉。从腹主静脉采集血液。采用自动血细胞分析仪进行血常规检查，采用角氨酸氧化酶法测定超氧化物歧化酶（SOD）活性。采用TBA测定脂质过氧化产物丙二醛（MDA），DTNB测定谷胱甘肽过氧化物酶（GSH-Px），观察不同麻醉方法、不同麻醉药物对小鼠红细胞免疫功能的影响。结果:水合氯醛腹腔注射组Het水平显着高于其他三组(P
Objective: To explore effect of different anesthesia methods and different anesthetics on erythrocyte immune function in mice. Methods: The mice were anesthetized by isoflurane and ether inhalation, and also under intraperitoneal anesthesia with sodium pentobarbital and chloral hydrate. Blood was collected from the ventro-cardinal vein. Automatic blood cell analyzer was used for routine blood examination, and the canthine oxidase method was used to measure the superoxide dismutase (SOD) activity. Lipid peroxidation product malondialdehyde (MDA) was measured with TBA, and glutathione peroxidase (GSH-Px) was measured with DTNB, and then the effect of different anesthesia methods and different anesthetics on erythrocyte immune function in mice was observed. Results: Het level of chloral hydrate intraperitoneal injection group was significantly higher than the other three groups (P