SINGLE-STRAND CLEAVAGE OF DNA BY CU(II) AND THIOLS - A POWERFUL CHEMICAL DNA-CLEAVING SYSTEM
SINGLE-STRAND CLEAVAGE OF DNA BY CU(II) AND THIOLS - A POWERFUL CHEMICAL DNA-CLEAVING SYSTEM
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DOI：
10.1016/0006-291x(89)90788-2
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发表时间：
1989-08-15
影响因子：
3.1
通讯作者：
DOUGLAS, KT
中科院分区：
文献类型：
--
作者：
REED, CJ;DOUGLAS, KT
还原的硫辛酸，在铜离子的存在下，引入单链切口pSP64质粒DNA在微摩尔浓度，转换成开放的圆形，并最终，线性形式的超螺旋。研究了反应的金属离子特异性，并且在Cu 2+、Co 2+、Cr 3+、Fe 3+、Fe 2+、Ni 2+、Mn 2+和Zn 2+中，只有Cu 2+离子在这些低浓度下是硫醇诱导的DNA切割的催化剂。广泛的硫醇和二硫醇被发现在Cu 2+离子的存在下作为DNA切割剂是有活性的。
Reduced lipoic acid, in the presence of cupric ions, introduced single-strand nicks into pSP64 plasmid DNA at micromolar concentrations, converting the supercoiled into open circular and, eventually, linear forms. The metal ion specificity of the reaction was investigated and, of Cu2+, Co2+, Cr3+, Fe3+, Fe2+, Ni2+, Mn2+ and Zn2+, only Cu2+ ions were catalysts for the thiol-induced DNA cleavage at these low concentrations. A wide range of thiols and dithiols was found to be active as DNA cleavers in the presence of Cu2+ ions.