Identification of polymorphonuclear leukocyte and HL-60 cell receptors for adhesins of Streptococcus gordonii and Actinomyces naeslundii
Identification of polymorphonuclear leukocyte and HL-60 cell receptors for adhesins of Streptococcus gordonii and Actinomyces naeslundii
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DOI：
10.1128/iai.68.11.6346-6354.2000
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发表时间：
2000-11-01
影响因子：
3.1
通讯作者：
Sandberg, AL
中科院分区：
文献类型：
--
作者：
Ruhl, S;Cisar, JO;Sandberg, AL
口腔链球菌和放线菌与多形核白细胞（PMNs）的相互作用，分别由唾液酸和半乳糖/半乳糖NAc反应性粘附素介导，导致PMNs的激活，从而可能导致口腔炎症的发生。唾液酸酶处理的中性粒细胞或HL-60细胞废除的粘附链球菌gordonii，但需要的粘附放线菌naeslundii，同样的效果，这些细菌粘附到一个主要的150 kDa的表面糖蛋白的中性粒细胞或未分化的HL-60细胞和分化的HL-60细胞的130 kDa的表面糖蛋白。这些糖蛋白都被确定为白唾液酸（CD 43）的免疫沉淀与一个特定的单克隆抗体（MAb），链球菌和放线菌粘附到一个200 kDa的小PMN表面糖蛋白也检测到细菌覆盖的未处理和唾液酸酶处理的硝酸纤维素转移，分别。用特异性单克隆抗体免疫沉淀法鉴定该糖蛋白为白细胞共同抗原（CD 45）。CD 43和CD 45都具有细胞外粘蛋白样结构域，除了涉及信号转导的细胞内结构域之外，因此，链球菌和放线菌与这些哺乳动物细胞表面糖蛋白的粘蛋白样结构域的相互作用不仅导致粘附，而且还可能代表这些细菌激活PMN的初始步骤。
Interactions of oral streptococci and actinomyces with polymorphonuclear leukocytes (PMNs), mediated by sialic acid- and Gal/GalNAc-reactive adhesins, respectively, result in activation of the PMNs and thereby may contribute to the initiation of oral inflammation. Sialidase treatment of PMNs or HL-60 cells abolished adhesion of Streptococcus gordonii but was required for adhesion of Actinomyces naeslundii, The same effects of sialidase were noted for adhesion of these bacteria to a major 150-kDa surface glycoprotein of either PMNs or undifferentiated HL-60 cells and to a 130-kDa surface glycoprotein of differentiated HL-60 cells. These glycoproteins were both identified as leukosialin (CD43) by immunoprecipitation with a specific monoclonal antibody (MAb), Adhesion of streptococci and actinomyces to a 200-kDa minor PMN surface glycoprotein was also detected by bacterial overlay of untreated and sialidase-treated nitrocellulose transfers, respectively. This glycoprotein was identified as leukocyte common antigen (CD45) by immunoprecipitation with a specific MAb. CD43 and CD45 both possess extracellular mucinlike domains in addition to intracellular domains that are implicated in signal transduction, Consequently, the interactions of streptococci and actinomyces with the mucinlike domains of these mammalian cell surface glycoproteins result not only in adhesion but, in addition, may represent the initial step in PMN activation by these bacteria.