Immunization with pneumolysin protects against both retinal and global damage caused by Streptococcus pneumoniae endophthalmitis.
Immunization with pneumolysin protects against both retinal and global damage caused by Streptococcus pneumoniae endophthalmitis.
复制标题
肺炎链球菌溶血素免疫可防止肺炎链球菌眼内炎引起的视网膜和整体损伤。
DOI：
10.1089/jop.2010.0077
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Marquart,MaryE
中科院分区：
文献类型：
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作者：
Sanders,MelissaE;Norcross,ErinW;Moore3rd,QuincyC;Fratkin,Jonathan;Thompson,Hilary;Marquart,MaryE
目的：确定肺炎球菌溶血素（Pneumolysin，Pneumolysin）免疫新西兰白色家兔是否能预防肺炎球菌眼内炎（Pneumococcal endophthalmitis.Methods），用Pneumolysin的突变体免疫新西兰白兔，直到血清IgG滴度≥ 51，200时，Pneumolysin的细胞溶解活性仅保留1%。对于阴性对照，用磷酸盐缓冲盐水（模拟）免疫家兔。每个玻璃体注射102个菌落形成单位的肺炎链球菌临床眼内炎分离株。在感染后24和48 h（PI）通过裂隙灯检查对眼内炎的严重程度进行分级。结果：免疫兔在感染后24和48 h的裂隙灯检查评分均显著低于假免疫兔（P≤ 0.002）。PI 24或48 h玻璃体中的细菌载量无显著差异。组织学切片显示，模拟免疫兔的视网膜似乎被破坏，而模拟免疫兔的视网膜基本上保持完整。到PI 48 h，损伤扩散到模拟免疫兔的房水、基质和结膜。在免疫兔的玻璃体中观察到最小的损伤，并且没有扩散到眼睛的其他部分。结论：肺炎球菌性眼内炎对兔眼的损伤有一定的保护作用。
Purpose:To determine whether immunization with pneumolysin (PLY) protects against pneumococcal endophthalmitis.Methods:New Zealand white rabbits were immunized with a mutant form of PLY that retains only 1% of its cytolytic activity until serum IgG titers were ≥51,200. For a negative control, rabbits were immunized with phosphate-buffered saline (mock). Each vitreous was injected with 102colony-forming units of a clinical endophthalmitis isolate ofStreptococcus pneumoniae. Severity of endophthalmitis was graded by slit lamp examination at 24 and 48 h postinfection (PI). Serial dilutions of vitreous were plated for bacterial colony-forming units quantitation, eyes were extracted for histology, and a whole blood survival assay was performed.Results:Immunized rabbits had a significantly lower mean slit lamp examination score at 24 and 48 h PI when compared to mock immunized rabbits (P≤ 0.002). There was not a significant difference in bacterial load in the vitreous at 24 or 48 h PI. Histological sections showed that retinas of mock immunized rabbits appeared to be destroyed, whereas those of PLY immunized rabbits remained largely intact. Damage spread to the aqueous humor, stroma, and conjunctiva of mock immunized rabbits by 48 h PI. Minimal damage was observed in the vitreous of PLY immunized rabbits and did not spread to other parts of the eye. Whole blood from immunized rabbits inhibited the growth of bacteria better than whole blood from mock immunized rabbits.Conclusion:Immunization with PLY helps protect the eye from damage caused by pneumococcal endophthalmitis.