Early cellular changes of human mesenchymal stem cells and their interaction with other cells
Early cellular changes of human mesenchymal stem cells and their interaction with other cells
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DOI：
10.1111/j.1067-1927.2005.130411.x
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发表时间：
2005-07-01
影响因子：
2.9
通讯作者：
Akita, S
中科院分区：
文献类型：
--
作者：
Akino, K;Mineda, T;Akita, S
研究了人间充质干细胞与潜在的相邻细胞如内皮细胞、真皮成纤维细胞和表皮角质形成细胞之间的细胞间相互作用。使用8 μ m孔的改良双Boyden室试验显示，在孵育16小时期间，人间充质干细胞向人表皮角质形成细胞的趋化细胞迁移比其他细胞，如脐动脉内皮细胞和真皮成纤维细胞更强（分别为336.2 +/- 52.33、36.0 +/- 11.20和62.7 +/- 18.16，细胞/视野，p < 0.01;内皮细胞和角质形成细胞之间以及成纤维细胞和角质形成细胞之间的比较）。扫描电子显微镜显示人间充质干细胞通过孔迁移，在下室中有内皮细胞、成纤维细胞或角质形成细胞。间充质干细胞超微结构的变化发生，包括一个更大的常染色质核，当细胞被放置在培养基中含有10%的胎牛血清，而碱性成纤维细胞生长因子保持不成熟的细胞形态4天。单层共培养也显示了表皮角质形成细胞附近的人间充质干细胞超微结构形态的变化。这些数据表明，人间充质干细胞可能与人表皮角质形成细胞相互作用，以加速伤口愈合和覆盖。
Cell-to-cell interactions between human mesenchymal stem cells and potential adjacent cells such as endothelial cells, dermal fibroblasts, and epidermal keratinocytes was investigated. A modified dual Boyden chamber assay using 8-mu m pores revealed a more powerful chemotactic cell migration of human mesenchymal stem cells toward human epidermal keratinocytes than other cells, such as umbilical artery endothelial cells and dermal fibroblasts, during 16 hours of incubation (336.2 +/- 52.33, 36.0 +/- 11.20, and 62.7 +/- 18.16, cells/field, respectively, p < 0.01; comparison between endothelial cells and keratinocytes, and fibroblasts and keratinocytes). Scanning electron microscopy showed human mesenchymal stem cell migration through the pores, with endothelial cells, fibroblasts, or keratinocytes in the lower chambers. Mesenchymal stem cell ultrastructural changes occurred, including a larger euchromatin nucleus, when the cells were placed in medium containing 10 percent fetal bovine serum, whereas basic fibroblast growth factor maintained the immature cell morphology for 4 days. Monolayer coculture also showed human mesenchymal stem cell changes in ultrastructural morphology in the vicinity of the epidermal keratinocytes. These data suggest that human mesenchymal stem cells may interact with human epidermal keratinocytes to accelerate wound healing and coverage.