Double-stranded RNA transcribed from vector-based oligodeoxynucleotide acts as transcription factor decoy
Double-stranded RNA transcribed from vector-based oligodeoxynucleotide acts as transcription factor decoy
复制标题
从基于载体的寡脱氧核苷酸转录的双链RNA充当转录因子诱饵
DOI：
10.1016/j.bbrc.2014.12.091
复制
发表时间：
2015-02-06
影响因子：
3.1
通讯作者：
Liu, Zhiguo
中科院分区：
文献类型：
--
作者：
Xiao, Xiao;Gang, Yi;Liu, Zhiguo
在本研究中，我们设计了一种基于短发夹状RNA载体的寡核苷酸(VB-ODN)，它携带转录因子(TF)的共同序列，可以作为诱饵来阻断TF的活性。具体地说，核因子-kappa B的VB-ODN可以抑制HEK293细胞的细胞活力和下游基因的表达，但不影响核因子-kappa B的表达。凝胶迁移率改变实验证实VB-ODN产生的双链RNA与核因子-kappaB特异性结合。此外，为另外三个转录因子设计的类似的VB-ODN也抑制了它们下游的基因表达，但不抑制自身的基因表达。本研究为阻断转铁蛋白活性提供了一种新的诱饵设计。(C)2014 Elsevier Inc.保留所有权利。
In this study, we designed a short hairpin RNA vector-based oligodeoxynucleotide (VB-ODN) carrying transcription factor (TF) consensus sequence which could function as a decoy to block TF activity. Specifically, VB-ODN for Nuclear factor-kappa B (NF-kappa B) could inhibit cell viability and decrease downstream gene expression in HEK293 cells without affecting expression of NF-kappa B itself. The specific binding between VB-ODN produced double-stranded RNA and NF-kappa B was evidenced by electrophoretic mobility shift assay. Moreover, similar VB-ODNs designed for three other TFs also inhibit their downstream gene expression but not that of themselves. Our study provides a new design of decoy for blocking TF activity. (C) 2014 Elsevier Inc. All rights reserved.