Potassium-induced release of endothelium-derived relaxing factor from canine femoral arteries.
Potassium-induced release of endothelium-derived relaxing factor from canine femoral arteries.
复制标题
钾诱导犬股动脉释放内皮源性舒张因子。
DOI：
10.1161/01.res.62.6.1098
复制
发表时间：
1988
影响因子：
20.1
通讯作者：
Vanhoutte,PM
中科院分区：
文献类型：
--
作者：
Rubanyi,GM;Vanhoutte,PM
在生物测定系统中设计实验以分析升高的（从5.9mM至7.5- 45.9mM）细胞外K+浓度对内皮源性舒张因子释放的影响。将具有内皮的犬股动脉节段（供体节段）固定在器官浴中，并用改良的Krebs-Ringer碳酸氢盐溶液灌注;供体节段的流出物用于灌注不具有内皮的犬冠状动脉环（生物测定组织）。灌注液K+浓度升高1.6-15 mM，通过在供体节段上游管腔内输注氯化钾引起与前列腺素F2 α收缩的生物测定环的进一步收缩。相比之下，当将增加浓度的氯化钾（10-40 mM）以腔外方式添加到安装灌注节段的器官浴中时，生物测定环逐渐松弛。在生物测定环中，苯肾上腺素或前列腺素F2 α的腔外应用未引起松弛。从供体部分去除内皮或选择性暴露的部分（但不是生物测定环），以Ca 2+缺乏的解决方案防止K+诱导的松弛。用已知内源性血管活性物质（乙酰胆碱、去甲肾上腺素、腺嘌呤核苷酸和前列腺素）的抑制剂处理供体节段和生物测定环，对氯化钾腔外应用引起的生物测定环松弛无显著影响。同时测量供体节段和生物测定环的等长力变化表明，K+浓度的腔外升高也使节段松弛，并且同时腔内输注氯化钾不能防止松弛。（250字处删节）
Experiments were designed in a bioassay system to analyze the effect of elevated (from 5.9 mM to 7.5-45.9 mM) extracellular K+ concentration on the release of endothelium-derived relaxing factor. Segments of canine femoral artery with endothelium (donor segment) were mounted in an organ bath and perfused with modified Krebs-Ringer bicarbonate solution; the effluent from the donor segment was used to superfuse a canine coronary artery ring without endothelium (bioassay tissue). Elevation of perfusate K+ concentration by 1.6-15 mM by intraluminal infusion of potassium chloride upstream of the donor segment evoked further contractions of bioassay rings contracted with prostaglandin F2 alpha. In contrast, the bioassay rings progressively relaxed when increasing concentrations of potassium chloride (10-40 mM) were added extraluminally to the organ bath where the perfused segment was mounted. Extraluminal application of phenylephrine or prostaglandin F2 alpha did not evoke relaxations in the bioassay ring. Removal of the endothelium from the donor segment or selective exposure of the segment (but not the bioassay ring) to Ca2+-deficient solution prevented the K+-induced relaxations. Treatment of the donor segment and the bioassay ring with inhibitors of known endogenous vasoactive substances (acetylcholine, norepinephrine, adenine nucleotides, and prostanoids) had no significant effect on the relaxation of the bioassay ring evoked by extraluminal application of potassium chloride. Simultaneous measurements of changes in isometric force in the donor segment and bioassay ring revealed that extraluminal elevation of K+ concentration relaxed the segments as well and that the relaxations could not be prevented by simultaneous intraluminal infusion of potassium chloride.(ABSTRACT TRUNCATED AT 250 WORDS)