Antifungal activity and the potential correlation with statin-producing ability: an optimized screening applied to filamentous fungi from Las Yungas subtropical rainforest.
Antifungal activity and the potential correlation with statin-producing ability: an optimized screening applied to filamentous fungi from Las Yungas subtropical rainforest.
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DOI：
10.3923/jm.2010.833.848
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发表时间：
2010-01-01
期刊：
影响因子：
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通讯作者：
Farina, J. I.
中科院分区：
文献类型：
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作者：
Cabral, M. E.;Delgado, O. D.;Farina, J. I.
研究的目的是调查从Las Yungas Pedemontana森林分离的真菌标本的他汀类药物相关的抗真菌活性。考虑到他汀类药物作为自然环境中竞争的潜在机制的参与，特别分析了其生产。遵循“去复制”的概念来鉴定天然产物，通过酵母生长抑制生物测定、TLC和RP-HPLC分析来筛选来自真菌分离物的提取物。根据三种替代方案进行真菌培养物的提取：玻璃珠、Waring搅拌机破碎或冻融法。Waring-blender-获得的真菌提取物导致潜在的他汀类药物相关化合物的产量高于玻璃珠破碎或冻融。在201株Las Yungas分离株中，约有10%显示出与他汀类药物相容的TLC（+）谱，因此被预选为潜在的他汀类药物生产者。在酵母生物测定中，13种提取物对S.酿酒酵母ATCC 32051的生长，而7个对产朊假丝酵母Pr 1 -2具有相同的效果。RP-HPLC显示的一些峰对应于已知的他汀类药物，如普伐他汀、洛伐他汀和辛伐他汀。他汀产生菌株被鉴定为肉座菌属和青霉属成员。尽管他汀类药物滴度较低（3 mg L-1），可能与非最佳生产条件有关，但一些真菌提取物能够抑制HMGCoA-还原酶1.3%至18.6%。因此，所选菌株的抗真菌活性可能与他汀类药物的产生有关，可能是自然生态系统中的竞争技能。这份报告强调了隐藏的生物技术潜力，如他汀类药物生产能力，本土丝状真菌从亚热带雨林的拉斯永加斯。此外，他汀类药物生产评估的优化方案，在此提出了未来的筛选程序。
Research was aimed at investigating statin-related antifungal activity of fungal specimens isolated from Las Yungas Pedemontana Forest. Considering the participation of statins as a potential mechanism for competition in natural environments, their production was particularly analyzed. Following the 'dereplication' concept for identifying natural products, extracts from fungal isolates were screened by yeast-growth inhibition bioassays, TLC and RP-HPLC analysis. Extraction from fungal cultures was performed according to three alternative protocols: glass-beads, Waring blender disruption, or a freeze-thawing method. Waring-blender-obtained fungal extracts led to higher yields of potentially statin-related compounds than those of glass-beads disruption or freeze-thawing. Around a 10% of 201 Las Yungas isolates showed statin-compatible TLC(+) profiles, thus being pre-selected as potential statin-producers. During yeast bioassays, 13 extracts inhibited S. cerevisiae ATCC 32051 growth whilst seven had the same effect on Candida utilis Pr1-2. Some peaks revealed by RP-HPLC corresponded to known statins like pravastatin, lovastatin and simvastatin. Statin-producing isolates were identified as Hypocrea and Penicillium genera members. Despite the low statin titres (3 mg L-1), likely related to non-optimal production conditions, some fungal extracts were able to inhibit HMGCoA-reductase between 1.3 and 18.6%. Antifungal activities of selected isolates could be thus related to statin production, probably as a competition skill in natural ecosystems. This report highlights the hidden biotechnological potential, such as statin production ability, of indigenous filamentous fungi from subtropical rainforests of Las Yungas. Additionally, optimized protocols for statin-production assessment are herein proposed for future screening programs.