OVER-EXPRESSION OF THE S13 RIBOSOMAL-PROTEIN IN ACTIVELY GROWING CELLS
OVER-EXPRESSION OF THE S13 RIBOSOMAL-PROTEIN IN ACTIVELY GROWING CELLS
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DOI：
10.1002/ijc.2910550218
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发表时间：
1993-09-09
影响因子：
6.4
通讯作者：
LUSTENBERGER, P
中科院分区：
文献类型：
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作者：
DENIS, MG;CHADENEAU, C;LUSTENBERGER, P
为了确定侵袭性的生物标志物，用2个不同致瘤性的大鼠结肠癌细胞系克隆了结肠癌中与正常上皮细胞相比高表达的基因。将一个进展型大鼠结肠癌克隆(PROB)cDNA文库与从这些PROB细胞或来自同一肿瘤的退化细胞(REGb)制备的mRNA合成的~(32)P-cDNADNA杂交。在初步筛选后，分离到了几个克隆。用RNA印迹法鉴定各克隆的特异性。其中一种(B9)被发现与ProB细胞中比正常成年大鼠结肠中丰富30倍的信使核糖核酸杂交，因此被选作进一步研究。Southern印迹分析未检测到基因的扩增，表明该基因转录水平的差异很可能是由于该基因转录增加所致。序列分析表明，该基因与大鼠S13核糖体蛋白的同源性约为98%。测定了该基因在一系列不同生长速率的大鼠细胞系中的表达水平。这两个参数之间有很好的相关性。我们的数据提示S13核糖体蛋白基因可以用来评估肿瘤细胞的生长速度，这可能与肿瘤细胞的侵袭性有关。在一项初步的实验中，用原位杂交法在一系列人类大肠肿瘤中检测到了S13核糖体蛋白的mRNA。在所分析的4个肿瘤中均可见强信号。(C)1993年Wiley-Liss，Inc.
In order to define biological markers of aggressiveness, 2 rat colon-carcinoma cell lines differing by their tumorigenicity were used to clone genes over-expressed in colon carcinoma as compared with normal epithelial cells. A progressive rat colon-carcinoma clone (PROb) cDNA library was hybridized with 32P-cDNA synthesized from mRNA prepared from these PROb cells, or from regressive cells (REGb) derived from the same tumor. Several clones were isolated after the initial screening. The specificity of each clone was confirmed by RNA blotting. One of these (B9) was found to hybridize to an mRNA 30-fold more abundant in PROb cells than in normal adult rat colon, and was therefore selected for further study. No gene amplification was detected by Southern blot analysis, indicating that the difference in mRNA content was most likely due to an increased transcription of this gene. Sequencing of the cDNA revealed approximately 98% homology with the rat S13 ribosomal protein. The expression level of this gene was determined in a series of rat cell lines with different growth rates. A good correlation was found between these 2 parameters. Our data suggest that the S13 ribosomal-protein gene can be used to evaluate the growth rate of tumor cells, which might be correlated with their aggressiveness. In an initial trial experiment, S13 ribosomal-protein mRNA was detected in a series of human colorectal tumors by in situ hybridization. A strong signal was seen in the 4 tumors analyzed. (C) 1993 Wiley-Liss, Inc.