Dimethyl sulfoxide induces oxidative stress in the yeast Saccharomyces cerevisiae
Dimethyl sulfoxide induces oxidative stress in the yeast Saccharomyces cerevisiae
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DOI：
10.1111/1567-1364.12091
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发表时间：
2013-12-01
影响因子：
3.2
通讯作者：
Bartosz, Grzegorz
中科院分区：
文献类型：
--
作者：
Sadowska-Bartosz, Izabela;Paczka, Aleksandra;Bartosz, Grzegorz
二甲基亚砜(DMSO)被用作包括酵母在内的细胞的冷冻保护剂，也被用作化合物的溶剂。我们报道了DMSO在酵母中诱导氧化应激。采用酿酒酵母WT菌株EG-103及其突变体SOD1Sod2和SOD1Sod2。酵母菌受到1-14%DMSO在28℃下作用1h，DMSO对酵母菌生长的抑制呈浓度依赖性，对缺乏SOD的突变株(尤其是SOD1Sod2)的抑制作用更为明显。细胞活力受到了损害。琥珀酸脱氢酶是一种对氧化胁迫敏感的FeS酶，其活性损失随DMSO浓度的增加而降低。DMSO促进了活性氧物种的形成，用二氢乙啶以浓度依赖的方式进行估计，这种影响在没有超氧化物歧化酶的突变体中再次表现得更加明显。细胞内谷胱甘肽含量随着DMSO浓度的增加而增加，这可能是一种代偿反应。DMSO降低了DPH的荧光偏振度，降低了膜流动性。这些结果表明，尽管DMSO通常被认为是抗氧化剂，但它会诱导酵母细胞的氧化应激。
Dimethyl sulfoxide (DMSO) is used as a cryoprotectant for the preservation of cells, including yeast, and as a solvent for chemical compounds. We report that DMSO induces oxidative stress in the yeast. Saccharomyces cerevisiae wt strain EG-103 and its mutants sod1, sod2, and sod1 sod2 were used. Yeast were subjected to the action of 1-14% DMSO for 1h at 28 degrees C. DMSO induced a concentration-dependent inhibition of yeast growth, the effect being more pronounced for mutants devoid of SOD (especially sod1 sod2). Cell viability was compromised. DMSO-concentration-dependent activity loss of succinate dehydrogenase, a FeS enzyme sensitive to oxidative stress, was observed. DMSO enhanced formation of reactive oxygen species, estimated with dihydroethidine in a concentration-dependent manner, the effect being again more pronounced in mutants devoid of superoxide dismutases. The content of cellular glutathione was increased with increasing DMSO concentrations, which may represent a compensatory response. Membrane fluidity, estimated by fluorescence polarization of DPH, was decreased by DMSO. These results demonstrate that DMSO, although generally considered to be antioxidant, induces oxidative stress in yeast cells.