Measurements of Carbon-14 With Cavity Ring-Down Spectroscopy.
Measurements of Carbon-14 With Cavity Ring-Down Spectroscopy.
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DOI：
10.1016/j.nimb.2015.05.036
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发表时间：
2015-10-15
期刊：
影响因子：
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通讯作者：
Turteltaub K
中科院分区：
文献类型：
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作者：
McCartt AD;Ognibene T;Bench G;Turteltaub K
加速器质谱（AMS）是生物样品中14 C定量的最灵敏的方法。在过去20年中，当需要非常高的灵敏度时，该技术已用于各种低剂量、人类健康相关的研究。AMS帮助开拓了这些科学方法，但其昂贵的设施和对训练有素的技术人员的要求限制了它们的扩散。通过腔衰荡光谱（CRDS）对14 C进行定量提供了一种方法，该方法消除了基于加速器的系统的许多缺点，并将补充AMS在生物医学研究中的使用。我们的初始原型，使用非理想波长的激光器，在次优的实验条件下，具有3.5现代，1-σ精度检测毫克大小，碳-14升高的样品。这些结果证明了原理的证明，并为开发能够具有生物相关灵敏度的光谱仪提供了起点。
Accelerator Mass Spectrometry (AMS) is the most sensitive method for quantitation of 14C in biological samples. This technology has been used in a variety of low dose, human health related studies over the last 20 years when very high sensitivity was needed. AMS helped pioneer these scientific methods, but its expensive facilities and requirements for highly trained technical staff have limited their proliferation. Quantification of 14C by cavity ring-down spectroscopy (CRDS) offers an approach that eliminates many of the shortcomings of an accelerator-based system and would supplement the use of AMS in biomedical research. Our initial prototype, using a non-ideal wavelength laser and under suboptimal experimental conditions, has a 3.5-modern, 1-σ precision for detection of milligram-sized, carbon-14-elevated samples. These results demonstrate proof of principle and provided a starting point for the development of a spectrometer capable of biologically relevant sensitivities.