Experimental chronic wasting disease (CWD) in the ferret
Experimental chronic wasting disease (CWD) in the ferret
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DOI：
10.1016/j.jcpa.2008.01.004
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发表时间：
2008-05-01
影响因子：
0.8
通讯作者：
Hoover, E. A.
中科院分区：
文献类型：
--
作者：
Sigurdson, C. J.;Mathiason, C. K.;Hoover, E. A.
慢性消耗病（CWD）是北美鹿、麋鹿和驼鹿的一种朊病毒疾病，影响自由放养和圈养的鹿科动物。慢性消耗病的潜在宿主范围仍不确定。通过脑内（IC）或口服（PO）途径给予感染性脑材料，实验研究了雪貂对CWD的易感性。在IC接种后15至20个月，雪貂出现了与朊病毒病一致的神经系统体征，包括多食、嗜睡、竖毛、脊柱前凸和共济失调。在雪貂适应CWD的第一次传代后，潜伏期缩短至5个月。海绵状改变在基底节、丘脑、中脑和脑桥脑最为明显。PrPCWD的沉积是颗粒状的，偶尔与神经元密切相关或位于神经元内。有没有斑块状或血管周围的PrP聚集体中看到CWD感染的鹿科动物。在蛋白质印迹中，PrPCWD糖型分布类似于鹿中的CWD，以显性二糖基化糖型为代表。雪貂CWD疾病后IC，但不是PO接种，即使经过31个月的观察。这些研究结果表明，CWD感染雪貂的显微镜和生化特征的CWD在鹿科动物，但似乎是相对抵抗口腔感染的主要CWD接种鹿的起源。(c)2008年由Elsevier Ltd.出版
Chronic wasting disease (CWD),a prion disease of North American deer, elk and moose affects both free-ranging and captive cervids. The potential host range for CWD remains uncertain. The susceptibility of the ferret to CWD was examined experimentally by administering infectious brain material by the intracerebral (IC) or oral (PO) route. Between 15 and 20 months after IC inoculation, ferrets developed neurological signs consistent with prion disease, including polyphagia, somnolence, piloerection, lordosis and ataxia. Upon first sub-passage of ferret-adapted CWD, the incubation period decreased to 5 months. Spongiform change in the neuropil was most marked in the basal ganglia, thalamus, midbrain and pons. The deposition of PrPCWD was granular and was occasionally closely associated with, or localized within, neurons. There were no plaque-like or perivascular PrP aggregates as seen in CWD-infected cervids. In western blots, the PrPCWD glycoform profile resembled that of CWD in deer, typified by a dominant diglycosylated glycoform. CWD disease in ferrets followed IC but not PO inoculation, even after 31 months of observation. These findings indicate that CWD-infected ferrets share microscopical and biochemical features of CWD in cervids, but appear to be relatively resistant to oral infection by primary CWD inoculum of deer origin. (c) 2008 Published by Elsevier Ltd.