A potent, water-soluble and photoinducible DNA cross-linking agent
A potent, water-soluble and photoinducible DNA cross-linking agent
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一种有效的水溶性光诱导 DNA 交联剂
DOI：
10.1021/ja029040o
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发表时间：
2003-02-05
影响因子：
15
通讯作者：
Weng, LH
中科院分区：
文献类型：
--
作者：
Wang, P;Liu, RP;Weng, LH
通过Mannich反应合成了水溶性DNA交联苯酚和双酚衍生物3和6。它们都可以通过可见光（波长> 400 nm）的光活化作用交联DNA。化合物6在pH5.0和7.7时能交联DNA，而在pH10.0时不能交联。密度泛函理论（DFT）计算表明，6具有扭曲结构。因此，它可以与DNA天然结合，并具有光活化后交联DNA的能力。
Water-soluble DNA cross-linking phenol and biphenol derivatives3and6have been synthesized by a Mannich reaction. Both of them can cross-link DNA by photoactivation using visible light (wavelength > 400 nm). Compound6can cross-link DNA at pH 5.0 and 7.7, whereas no cross-link was observed at pH 10.0. Density functional theory (DFT) calculation indicated that6displays a twist structure. Therefore, it could bind to DNA naturally and has potent property to cross-link DNA after photoactivation.