Microfluidic devices for the high-throughput chemical analysis of cells
Microfluidic devices for the high-throughput chemical analysis of cells
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DOI：
10.1021/ac0346510
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发表时间：
2003-11-01
影响因子：
7.4
通讯作者：
Ramsey, JM
中科院分区：
文献类型：
--
作者：
McClain, MA;Culbertson, CT;Ramsey, JM
报道了一种集成了细胞处理、快速细胞裂解、电泳分离和荧光胞质染料检测的微流体装置。使用载有荧光染料-羧基荧光素二乙酸酯、俄勒冈州绿色羧酸二乙酸酯或钙黄绿素AM的Jurkat细胞证明了装置功能。将加载的细胞从含细胞的储器流体动力学地运输到微流体装置上的区域，在该区域中它们被聚焦，然后使用电场快速裂解。完全裂解完成于
A microfluidic device is reported that integrated cell handling, rapid cell lysis, and electrophoretic separation and detection of fluorescent cytosolic dyes. The device function was demonstrated using Jurkat cells that were loaded with the fluorogenic dyes - carboxyfluorescein diacetate, Oregon green carboxylic acid diacetate, or Calcein AM. The loaded cells were hydrodynamically transported from the cell-containing reservoir to a region on the microfluidic device where they were focused and then rapidly lysed using an electric field. Complete lysis was accomplished in