Appearance of peroxidase reactivity within the rough endoplasmic reticulum of blood monocytes after surface adherence
Appearance of peroxidase reactivity within the rough endoplasmic reticulum of blood monocytes after surface adherence
复制标题
表面粘附后血单核细胞粗面内质网内过氧化物酶反应性的表现
DOI：
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发表时间：
1977
影响因子：
15.3
通讯作者：
D. Bainton
中科院分区：
文献类型：
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作者：
P. Bodel;B. Nichols;D. Bainton
兔血单核细胞，其中不含细胞化学可证明的过氧化物酶，开发过氧化物酶活性的RER和核周池后2小时内粘附到血清或纤维蛋白包被的表面。类似的反应性出现在表面粘附的人和大鼠血液单核细胞。在定位和特征方面，单核细胞中的这种酶反应性类似于通常在兔的常驻腹膜巨噬细胞以及其他物种中的几种类型的组织巨噬细胞中观察到的酶反应性。因此，这一观察结果支持了目前基于其他研究者的动力学数据的概念，即血液单核细胞是这种细胞的前体。此外，粘附后新酶活性的出现可能反映了质膜：表面相互作用引起的细胞代谢的改变。
Rabbit blood monocytes, which contain no cytochemically demonstrable peroxidase, develop peroxidatic activity in the RER and perinuclear cisternae within 2 h after adherence to serum- or fibrin-coated surfaces. A similar reactivity appears in surface-adherent human and rat blood monocytes. In both localization and characteristics, this enzyme reactivity in monocytes resembles that normally seen in the resident peritoneal macrophages of the rabbit, as well as in several types of tissue macrophages in other species. Thus this observation supports the concept, presently based on the kinetic data of other investigators, that blood monocytes are the precursors of such cells. Moreover, the appearance of new enzyme activity after adherence may reflect alterations in cellular metabolism resulting from plasma membrane:surface interactions.