PROTEINS SELECTIVELY RELEASED FROM WATER-EXTRACTED HUMAN ERYTHROCYTE-MEMBRANES UPON CITRACONYLATION OR MALEYLATION - ELECTROPHORETIC ANALYSIS AND CHROMATOGRAPHIC FRACTIONATION
PROTEINS SELECTIVELY RELEASED FROM WATER-EXTRACTED HUMAN ERYTHROCYTE-MEMBRANES UPON CITRACONYLATION OR MALEYLATION - ELECTROPHORETIC ANALYSIS AND CHROMATOGRAPHIC FRACTIONATION
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DOI：
10.1016/0005-2795(75)90033-1
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发表时间：
1975-01-01
期刊：
影响因子：
--
通讯作者：
LUNDAHL, P
中科院分区：
文献类型：
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作者：
LUNDAHL, P
1. 水提取的人红细胞膜（膜渣）的柠檬酸酰化或马来酰化（羧化）可溶解高达20%（w/w）的膜渣蛋白，但不到2%的磷脂。氨基酸分析和聚丙烯酰胺凝胶电泳表明，十二烷基硫酸盐存在下的增溶作用是选择性的。2. 在没有洗涤剂的情况下，聚丙烯酰胺凝胶电泳将溶解的酰化蛋白分离成四个分离良好的主区和两个或三个小区。两个主要区域含有高分子量的蛋白质（光谱成分），另外两个区域含有中等分子量的成分。3. 羟基磷灰石层析可以部分纯化中间分子量的溶解组分。4. 在pH 5和36℃的孵育条件下，经citraconylation溶解的未分离蛋白质的一些成分被特异性地降解或解离成亚基。
1. Citraconylation or maleylation (carboxylation) of water-extracted human erythrocyte membranes (membrane residue) solubilized up to 20%(w/w) of the membrane residue protein, but less than 2% of the phospholipids. Amino acid analysis and polyacrylamide gel electrophoresis in the presence of dodecylsulfate showed that the solubilization was selective. 2. Polyacrylamide gel electrophoresis in the absence of detergent separated the solubilized acylated proteins into four well-separated major zones and two or three minor zones. Two of the major zones contained proteins of high molecular weight (spectrin components) and the other two contained components of intermediate molecular weight. 3. The solubilized components of intermediate molecular weight could be partially purified by hydroxyapatite chromatography. 4. Upon incubation at pH 5 and 36 degrees C of the unfractionated proteins solubilized by citraconylation some components became specifically degraded or dissociated into subunits.