Assembly of lipoprotein (a) in transgenic rabbits expressing human apolipoprotein (a)
Assembly of lipoprotein (a) in transgenic rabbits expressing human apolipoprotein (a)
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DOI：
10.1006/bbrc.1999.0242
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发表时间：
1999-02-24
影响因子：
3.1
通讯作者：
Watanabe, T
中科院分区：
文献类型：
--
作者：
Fan, JL;Araki, M;Watanabe, T
由于载脂蛋白(A)[apo(A)]只在灵长类动物和人类中发现，因此由于缺乏合适的动物模型，对人脂蛋白(A)[Lp(A)]的研究一直受到阻碍。此外，转基因小鼠中的人载脂蛋白(A)不能与鼠载脂蛋白B结合形成Lp(A)颗粒。在这项研究中，我们产生了三只独立的转基因兔，在他们的血浆中以1.8-4.5 mg/dl的速度表达人载脂蛋白(A)。与转基因小鼠血浆不同，转基因兔血浆中约有80%的apo(A)与兔apo-B共价结合，并存在密度为1.02~1.10g/ml的级分，表明Lp(A)的形成。这些结果表明，表达人载脂蛋白(A)的转基因兔能够有效地组装Lp(A)，可作为研究人Lp(A)的动物模型。(C)1999年学术出版社。
The study of human lipoprotein (a) [Lp(a)] has been hampered due to the lack of appropriate animal models since apolipoprotein (a) [apo(a)] is found only in primates and humans. In addition, human apo(a) in transgenic mice can not bind to murine apoB to form Lp(a) particles. In this study, we generated three independent transgenic rabbits expressing human apo(a) in their plasma at 1.8-4.5 mg/dl. In the plasma of transgenic rabbits, unlike the plasma of transgenic mice, about 80% of the apo(a) was covalently associated with rabbit apo-B and was contained in the fractions with density 1.02-1.10 g/ml, indicating the formation of Lp(a). These results suggest that transgenic rabbits expressing human apo(a) exhibit efficient assembly of Lp(a) and can be used as an animal model for the study of human Lp(a). (C) 1999 Academic Press.