Cultivation of nitrite-dependent anaerobic methane-oxidizing bacteria: impact of reactor configuration
Cultivation of nitrite-dependent anaerobic methane-oxidizing bacteria: impact of reactor configuration
复制标题
亚硝酸盐依赖性厌氧甲烷氧化细菌的培养：反应器配置的影响
DOI：
10.1007/s00253-014-5835-z
复制
发表时间：
2014-09-01
影响因子：
5
通讯作者：
Xu, Xinhua
中科院分区：
文献类型：
--
作者：
Hu, Baolan;He, Zhanfei;Xu, Xinhua
依赖亚硝酸盐的厌氧甲烷氧化（n-damo）是一种由细菌介导的厌氧甲烷氧化与亚硝酸盐还原，是一种潜在的废水处理生物工艺。本文研究了反应器配置对n-damo细菌培养的影响。选择磁搅拌气举反应器（MSGLR）、序批式反应器（SBR）和连续搅拌槽式反应器（CSTR）进行细菌培养。采用定量PCR、16S rRNA基因测序、pmoA基因测序和荧光原位杂交（FISH）技术监测微生物群落。重点研究了底物抑制、甲烷传质和生物质冲蚀对三种反应器的影响。结果表明，MSGLR系统的总氮达摩活性和比氮达摩活性最高，是3种反应器系统中性能最好的。其最大体积氮去除率可达76.9 mg N L−1day−1，高于已有报道的5.1 ~ 37.8 mg N L−1d−1。
Nitrite-dependent anaerobic methane oxidation (n-damo) is mediated by bacteria that anaerobically oxidize methane coupled with nitrite reduction and is a potential bioprocess for wastewater treatment. In this work, the effect of reactor configuration on n-damo bacterial cultivation was investigated. A magnetically stirred gas lift reactor (MSGLR), a sequencing batch reactor (SBR), and a continuously stirred tank reactor (CSTR) were selected to cultivate the bacteria. Microbial community was monitored by using quantitative PCR, 16S rRNA gene sequencing,pmoA gene sequencing, and fluorescence in situ hybridization (FISH). The effects of substrate inhibition, methane mass transfer, and biomass washout in the three reactors were focused on. The results indicated that the MSGLR had the best performance among the three reactor systems, with the highest total and specific n-damo activities. Its maximum volumetric nitrogen removal rate was up to 76.9 mg N L−1day−1, which was higher than previously reported values (5.1–37.8 mg N L−1d−1).