Integration of a multichannel surface plasmon resonance sensor chip and refractive index matching film array for protein detection in human urine.
Integration of a multichannel surface plasmon resonance sensor chip and refractive index matching film array for protein detection in human urine.
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DOI：
10.1016/j.talanta.2022.123533
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发表时间：
2022-05
期刊：
影响因子：
6.1
通讯作者：
Honggang Wang;Huixiang Wang;Hao Zhang;Yafeng Huang;Nianrong Zhang;Wenge Li;Xianbo Qiu;Duli Yu;Lulu Zhang
中科院分区：
文献类型：
--
作者：
Honggang Wang;Huixiang Wang;Hao Zhang;Yafeng Huang;Nianrong Zhang;Wenge Li;Xianbo Qiu;Duli Yu;Lulu Zhang
大多数基于棱镜的表面等离子体共振（SPR）实验使用具有相似折射率的匹配流体将芯片安装在光学棱镜上。匹配流体的流动性容易影响光信号的传输。在本文中，集成SPR传感器芯片包括一个三层结构的流动层，金属层和折射率匹配层的演示，以解决有关的一致性和均匀性的问题。对匹配膜的杨氏模量、阵列间距、形状等参数进行了计算和优化。该芯片能够自粘附在光学棱镜上，有效避免气泡的产生。集成SPR传感器芯片的折射率检测灵敏度为3.4359 × 10− 6 RIU（折射率单位），芯片稳定时间得到有效缩短。集成的SPR传感器芯片还用于检测尿液样本中的kappa轻链蛋白和人血清白蛋白（HSA）。方法的检测限为0.06 μg/mL，而常规免疫比浊法的检测限为18.5 μg/mL。基于折射率匹配薄膜阵列的集成SPR传感器芯片在生物医学检测等领域具有巨大的应用潜力，包括床旁检测（POCT）。
Most prism-based surface plasmon resonance (SPR) experiments use matching fluid with a similar refractive index to mount the chip on the optical prism. The fluidity of the matching fluid easily affects the transmission of the optical signal. In this paper, an integrated SPR sensor chip comprises a three-layer structure of flow layer, metal layer and refractive index matching layer is demonstrated to address the problems related to consistency and uniformity. The Young's modulus, array spacing, shape and other parameters of the matching film were calculated and optimized. The chip can self-adhere to the optical prism, and effectively avoids the generation of air bubbles. The refractive index detection sensitivity of the integrated SPR sensor chip was 3.4359 × 10−6RIU (refractive index unit), and the chip stabilization time has been effectively shortened. The integrated SPR sensor chip was also used to detect kappa light chain protein and human serum albumin (HSA) in urine samples. The detection limit of kappa light chain protein was 0.06 μg/mL compared with 18.5 μg/mL by conventional immunoturbidimetry. The integrated SPR sensor chip based on refractive index matching film array has great potential in biomedical detection and other fields, including point-of-care testing (POCT).