A MIF-like effector suppresses plant immunity and facilitates nematode parasitism by interacting with plant annexins
A MIF-like effector suppresses plant immunity and facilitates nematode parasitism by interacting with plant annexins
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类 MIF 效应子通过与植物膜联蛋白相互作用抑制植物免疫并促进线虫寄生
DOI：
10.1093/jxb/erz348
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发表时间：
2019
影响因子：
6.9
通讯作者：
Heng Jian
中科院分区：
文献类型：
--
作者：
Jianlong Zhao;Lijuan Li;Qian Liu;Pei Liu;Shuang Li;Dan Yang;Yongpan Chen;Sophie Pagnotta;Bruno Favery;Pierre Abad;Heng Jian
植物寄生线虫分泌大量的效应器以促进寄生，但线虫效应器的详细功能及其植物靶标在很大程度上仍不清楚。在这里，我们描述了四种巨噬细胞移动抑制因子(MIF)在南方根结线虫中的特征，它们与人和动物寄生虫分泌的MIF相似。转录。数据显示，MiMIF在寄生过程中上调。免疫定位证明MiMIF蛋白从线虫皮下组织分泌到寄生虫表面，在植物组织和巨细胞中检测到。在植物中，MiMIFs RNA干扰在拟南芥中减少了侵染和线虫繁殖。瞬时表达MiMIF-2可抑制Bax和RBP1/Gpa2诱导的细胞死亡。MiMIF-2的异位表达导致拟南芥更高水平的敏感性，抑制flg22引发的免疫反应，并抑制H2O2诱导的[Ca2+]细胞内流。MiMIF-2相互作用蛋白的免疫沉淀、免疫共沉淀和双分子荧光互补验证揭示了MiMIF-2与两个拟南芥之间的特异性相互作用。膜联蛋白Annat1和Annat4参与钙离子的运输、胁迫反应和信号转导。这些膜联蛋白的表达或过度表达改变了线虫侵染。我们的结果提供了功能证据，证明线虫效应物从皮下分泌到寄生虫角质层表面的靶标蛋白和南方根结线虫利用MiMIF通过干扰膜联蛋白介导的植物免疫反应来促进寄生。
Plant-parasitic nematodes secrete numerous effectors to facilitate parasitism, but detailed functions of nematode effectors.and their plant targets remain largely unknown. Here, we characterized four macrophage migration inhibitory.factors (MIFs) in Meloidogyne incognita resembling the MIFs secreted by human and animal parasites. Transcriptional.data showed MiMIFs are up-regulated in parasitism. Immunolocalization provided evidence that MiMIF proteins are.secreted from the nematode hypodermis to the parasite surface, detected in plant tissues and giant cells. In planta.MiMIFs RNA interference in Arabidopsis decreased infection and nematode reproduction. Transient expression of.MiMIF-2 could suppress Bax- and RBP1/Gpa2-induced cell death. MiMIF-2 ectopic expression led to higher levels of.Arabidopsis susceptibility, suppressed immune responses triggered by flg22, and impaired [Ca2+]cyt influx induced by.H2O2. The immunoprecipitation of MiMIF-2-interacting proteins, followed by co-immunoprecipitation and bimolecular.fluorescence complementation validations, revealed specific interactions between MiMIF-2 and two Arabidopsis.annexins, AnnAt1 and AnnAt4, involved in the transport of calcium ions, stress responses, and signal transduction..Suppression of expression or overexpression of these annexins modified nematode infection. Our results provide.functional evidence that nematode effectors secreted from hypodermis to the parasite cuticle surface target host.proteins and M. incognita uses MiMIFs to promote parasitism by interfering with the annexin-mediated plant immune.responses.