Differential diagnosis and molecular characterization of Theileria spp. in sika deer (Cervus nippon) in Hokkaido, Japan
Differential diagnosis and molecular characterization of Theileria spp. in sika deer (Cervus nippon) in Hokkaido, Japan
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DOI：
10.1016/j.parint.2019.01.005
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发表时间：
2019-06-01
影响因子：
1.9
通讯作者：
Xuan, Xuenan
中科院分区：
文献类型：
--
作者：
Lee, Seung-Hun;Moumouni, Paul Franck Adjou;Xuan, Xuenan
梅花鹿（Cervus nippon）广泛分布于亚洲国家，是日本北海道最常见的野生动物之一。先前的研究发现了泰勒虫属（Theileria spp）。日本梅花鹿中包括泰勒虫属 (Theileria sp.)。 Thrivae 属于 T. cervi 组和 Theileria sp。索拉属于 T. capreoli 组。然而，这些研究未能在没有测序的情况下区分这两个物种。因此，日本北海道鹿泰勒虫病的流行病学信息有限。这项研究区分了两种泰勒虫属。使用限制性片段长度多态性（RFLP）。基于 PCR-RFLP，泰勒虫属 (Theileria spp)。在 117 个样本中的 103 个 (88.0%) 中发现了泰勒虫，每种寄生虫的患病率分别为 86.3% (n = 101) 和 57.3% (n = 67)。分别与 Thrivae 和 T. capreoli 类似。基于 18S rRNA 的系统发育分析表明泰勒虫属 (Theileria sp.) 与泰勒虫属 (Theileria sp.) 之间存在密切的亲缘关系。中国的 Thrivae 和 T. cervi。此外，内转录间隔区的系统发育分析也表明泰勒虫属与泰勒虫属之间存在密切的关系。 Thrivae 和 T. cervi。
Sika deer (Cervus nippon) is widely distributed in Asian countries and is one of the most common wildlife animals in Hokkaido, Japan. Previous studies identified Theileria spp. in sika deer in Japan including Theileria sp. Thrivae belonging to T. cervi group and Theileria sp. sola belonging to T. capreoli group. However, the studies failed to differentiate these two species without sequencing. Therefore, epidemiological information on cervine theileriosis in Hokkaido, Japan is limited. This study differentiated the two Theileria spp. using restriction fragments length polymorphism (RFLP). Based on the PCR-RFLP, Theileria spp. were identified in 103 (88.0%) of 117 samples, and the prevalence of each parasites were 86.3% (n = 101) and 57.3% (n = 67) for Theileria sp. Thrivae and T. capreoli-like, respectively. Phylogenetic analysis based on the 18S rRNA showed a close relationship between Theileria sp. Thrivae and T. cervi in China. In addition, phylogenetic analysis of internal transcribed spacer regions also showed a close relationship between Theileria sp. Thrivae and T. cervi.