Ag+ and cysteine detection by Ag+-guanine interaction based on graphene oxide and G-quadruplex DNA
Ag+ and cysteine detection by Ag+-guanine interaction based on graphene oxide and G-quadruplex DNA
复制标题
基于氧化石墨烯和 G-四链体 DNA 的 Ag-鸟嘌呤相互作用检测 Ag 和半胱氨酸
DOI：
10.1039/c2ay25362c
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发表时间：
2012-07-01
影响因子：
3.1
通讯作者：
Zhou, Xiang
中科院分区：
文献类型：
--
作者：
Liu, Lin;Liu, Wenting;Zhou, Xiang
Ag+ 和 Cys 检测的灵敏和选择性技术是基于氧化石墨烯 (GO) 和 G-四链体 DNA 设计的。 G-四联体结构可以从氧化石墨烯（GO）表面捕获荧光分子吖啶橙（AO），从而恢复最初被GO猝灭的AO荧光。然后Ag+的添加会将AO释放回GO，因为Ag+会破坏G-四联体结构，再次产生荧光猝灭过程。基于这种“关闭”原理，开发了一种高灵敏度和选择性的Ag+检测方法。此外，半胱氨酸（Cys）可以从DNA的鸟嘌呤碱基捕获Ag+，从而导致G-四链体DNA结构的再生，从而再生荧光信号。这种“开启”原理可用于开发高选择性和灵敏的半胱氨酸检测方法。
Sensitive and selective techniques for both Ag+ and Cys detection have been designed based on graphene oxide (GO) and G-quadruplex DNA. The G-quadruplex structure can capture the fluorescent molecule acridine orange (AO) from the surface of graphene oxide (GO), which will recover the fluorescence of AO that was initially quenched by GO. Then the addition of Ag+ will release AO back to GO, because Ag+ can destroy the G-quadruplex structure, giving a fluorescence quenching process again. Based on this "turn off" principle, a highly sensitive and selective Ag+ detection method was developed. In addition, cysteine (Cys) can capture Ag+ from the guanine base of DNA which will result in the regeneration of the G-quadruplex DNA structure and thus the regeneration of the fluorescence signal. This "turn on" principle can be used for the development of a highly selective and sensitive detection method for Cys.