Epidermal growth factor (EGF) stimulates EGF receptor synthesis.
Epidermal growth factor (EGF) stimulates EGF receptor synthesis.
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发表时间：
1986-04
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影响因子：
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通讯作者：
H. S. Earp§V;K. Austin;Joyce BlaisdellSg;R. A. Rubin;Karen G NelsonII;Lester W. LeelJ;Joe W. GrishamSJJ
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文献类型：
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作者：
H. S. Earp§V;K. Austin;Joyce BlaisdellSg;R. A. Rubin;Karen G NelsonII;Lester W. LeelJ;Joe W. GrishamSJJ
表皮生长因子（EGF）结合到特异性170，000-道尔顿跨膜糖蛋白的胞外结构域;这导致从细胞表面快速去除配体和受体。在WB细胞（大鼠肝上皮细胞系）中，配体导向的受体内化导致受体降解。我们测试了EGF受体是否在EGF结合后以组成型或增强的速率补充，通过免疫沉淀来自与[35 S]蛋氨酸培养的细胞的生物合成标记的EGF受体。EGF刺激受体合成2小时内以剂量依赖性的方式，这是特别明显的，当检查新生形式的受体。为了确定EGF作用的位点，电泳后将总WB细胞RNA转移到硝酸纤维素纸上，并与来自人EGF受体的外部和细胞质编码区的cDNA探针杂交。EGF使受体mRNA增加3-5倍。因此，至少在某些细胞中，导致EGF受体降解的EGF表面作用被对受体合成的积极作用所抵消。
Epidermal growth factor (EGF) binds to the extracellular domain of a specific 170,000-dalton transmembrane glycoprotein; this results in rapid removal of both ligand and receptor from the cell surface. In WB cells, a rat hepatic epithelial cell line, ligand-directed receptor internalization leads to receptor degradation. We tested whether the EGF receptor was replenished at a constitutive or enhanced rate following EGF binding by immunoprecipitating biosynthetically labeled EGF receptor from cells cultured with [35S]methionine. EGF stimulated receptor synthesis within 2 h in a dose-dependent manner; this was particularly evident when examining the nascent form of the receptor. To determine the site of EGF action, total WB cell RNA was transferred to nitrocellulose paper after electrophoresis and was hybridized to cDNA probes from both the external and cytoplasmic coding regions of the human EGF receptor. EGF increased receptor mRNA by 3-5-fold. Therefore, at least in some cells, the surface action of EGF that leads to EGF receptor degradation is counterbalanced by a positive effect on receptor synthesis.