A study on the bioactivity of chitosan/nano-hydroxyapatite composite scaffolds for bone tissue engineering
A study on the bioactivity of chitosan/nano-hydroxyapatite composite scaffolds for bone tissue engineering
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DOI：
10.1016/j.eurpolymj.2006.08.009
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发表时间：
2006-12-01
影响因子：
6
通讯作者：
Zhang, Xiufang
中科院分区：
文献类型：
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作者：
Kong, Lijun;Gao, Yuan;Zhang, Xiufang
为了提高壳聚糖的生物相容性和生物活性，将羟基磷灰石原位复合制成壳聚糖支架。通过模拟体液培养，检测复合支架上形成的磷灰石及其上培养的成骨前细胞的活性，研究复合支架的生物活性。采用扫描电镜（SEM）、x射线衍射（XRD）、傅里叶变换红外光谱（FTIR）和重量测量对磷灰石层进行了评价。复合分析表明，两种支架在模拟体液中孵育后均形成碳酸盐羟基磷灰石。随着复合材料中纳米羟基磷灰石含量的增加，在支架上形成的磷灰石数量增加。与纯壳聚糖相比，纳米羟基磷灰石复合材料在仿生过程中更容易形成磷灰石，表明复合材料具有更好的矿化活性。此外，在磷灰石包被支架上培养的成骨前细胞表现出不同的行为。细胞在磷灰石包被复合支架上的增殖优于壳聚糖包被磷灰石支架。此外，对支架上培养的细胞在条件培养基中碱性磷酸酶活性进行了测定。复合支架上的细胞显示出较高的碱性磷酸酶活性，表明细胞分化水平较高。结果表明，纳米羟基磷灰石的加入提高了壳聚糖/纳米羟基磷灰石复合支架的生物活性。另一方面，即衬底的组成会影响其表面磷灰石的形成，预加载羟基磷灰石可以增强磷灰石涂层。这对骨组织工程中磷灰石涂层聚合物支架的制备具有重要意义。(c) 2006 Elsevier Ltd.版权所有。
In order to increase the biocompatibility and bioactivity of chitosan, hydroxyapatite had been in situ combined into chitosan scaffolds. The bioactivity of the composite scaffolds was studied by examining the apatite formed on the scaffolds by incubating in simulated body fluid and the activity of preosteoblasts cultured on them. The apatite layer was assessed using scanning electronic microscope (SEM), X-ray diffraction (XRD), Fourier-Transformed Infrared spectroscopy (FTIR) and weight measurement. Composite analysis showed that after incubation in simulated body fluid on both of the scaffolds carbonate hydroxyapatite was formed. With increasing nano-hydroxyapatite content in the composite, the quantity of the apatite formed on the scaffolds increased. Compared with pure chitosan, the composite with nano-hydroxyapatite could form apatite more readily during the biomimetic process, which suggests that the composite possessed better mineralization activity. Furthermore, preosteoblast cells cultured on the apatite-coated scaffolds showed different behavior. On the apatite-coated composite scaffolds cells presented better proliferation than on apatite-coated chitosan scaffolds. In addition, alkaline phosphatase activities of cells cultured on the scaffolds in conditioned medium were assessed. The cells on composite scaffolds showed a higher alkaline phosphatase activity which suggested a higher differentiation level. The results indicated that the addition of nano-hydroxyapatite improved the bioactivity of chitosan/nano-hydroxyapatite composite scaffolds. On the other hand, that is to say composition of substrates could affect the apatite formation on them, and pre-loaded hydroxyapatite can enhance the apatite-coating. It will also be significant in preparation of apatite-coating polymer scaffolds for bone tissue engineering. (c) 2006 Elsevier Ltd. All rights reserved.