Validation of a phenol-sulfuric acid method in a microplate format for the quantification of soluble sugars in ruminant feeds
Validation of a phenol-sulfuric acid method in a microplate format for the quantification of soluble sugars in ruminant feeds
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微孔板形式的苯酚-硫酸法对反刍动物饲料中可溶性糖定量的验证
DOI：
10.1111/asj.13530
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发表时间：
2021
影响因子：
2
通讯作者：
N.
中科院分区：
文献类型：
--
作者：
Kondo;M.;Mulianda;R.;Matamura;M.;Shibata;T.;Mishima;T.;Jayanegara;A.;Isono;N.
饲料中的可溶性糖对于反刍动物的生产很重要；然而，在短时间内进行大量的糖分析是一项艰巨的任务。在这里，我们开发了一种微孔板形式的苯酚-硫酸 (PSA) 测定法，用于定量反刍动物饲料中的可溶性糖。该方法简单、快速，仅需要少量有害试剂。我们发现测定测量结果不受饲料中含有的代表性有机酸和糖醇的影响。在 PSA 测定之前用乙醇提取物处理活性炭可有效去除干扰化合物，从而更准确地测定某些饲料中的可溶性糖。此外，本方法的日间和日内重复性是可以接受的。因此，我们得出结论，本研究开发的方法适用于反刍动物饲料中可溶性糖含量的常规分析。
Soluble sugars in feeds are important for ruminant production; however, performing numerous sugar analyses within a short period is a laborious task. Here, we developed a phenol‐sulfuric acid (PSA) assay in a microplate format to quantify soluble sugars in ruminant feeds. This method is easy and quick and requires only a small quantity of harmful reagents. We found that assay measurements were not affected by the representative organic acids and sugar alcohol contained in feeds. The treatment of activated charcoal with ethanol extract prior to the PSA assay was effective in removing interfering compounds for a more accurate determination of soluble sugars in certain feeds. Furthermore, the inter‐day and intra‐day repeatability of the present method was acceptable. Hence, we conclude that the method developed in this study is suitable for routine analysis of soluble sugars content in ruminant feeds.