Measurement of mitochondrial ROS production.
Measurement of mitochondrial ROS production.
复制标题
线粒体ROS产生的测量。
DOI：
10.1007/978-1-60761-756-3_16
复制
发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Starkov, Anatoly A
中科院分区：
文献类型：
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作者：
Starkov, Anatoly A
活性氧（ROS）在许多疾病中作为加重或主要因素的重要性已被广泛认识，线粒体被认为是细胞内ROS的主要来源。在动物或培养细胞中常规测量线粒体活性氧还不可能有一定程度的确定性。然而，在离体线粒体水平上，可以很容易地监测和量化ROS的产生速率，确定ROS产生的主要位点，并比较正常和病变组织分离的线粒体中ROS的产生速率。在本章中，我们详细描述了最新和可靠的方法来测量线粒体ROS作为H2O2排放率。这种方法可以用最小的修改来测量从各种组织中分离的线粒体在各种实验条件下产生的H2O2。
The significance of reactive oxygen species (ROS) as aggravating or primary factors in numerous pathologies is widely recognized, with mitochondria being considered the major intracellular source of ROS. It is not yet possible to routinely measure mitochondrial ROS in animals or cultured cells with a reasonable degree of certainty. However, at the level of isolated mitochondria, one can easily monitor and quantify the rate of ROS production, identify major sites of ROS production, and compare the rates of ROS production in mitochondria isolated from normal and diseased tissue. In this chapter, we describe in detail the most recent and reliable method to measure mitochondrial ROS as the rate of H2O2 emission. This method may be employed with minimal modifications to measure H2O2 production by mitochondria isolated from various tissues and under a wide variety of experimental conditions.