A phylogenetic assessment of the eukaryotic light-harvesting antenna proteins, with implications for plastid evolution
A phylogenetic assessment of the eukaryotic light-harvesting antenna proteins, with implications for plastid evolution
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DOI：
10.1007/pl00006445
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发表时间：
1999-01-01
影响因子：
3.9
通讯作者：
Green, BR
中科院分区：
文献类型：
--
作者：
Durnford, DG;Deane, JA;Green, BR
捕光复合体(LHC)是一个存在于所有光合作用真核生物中的叶绿素结合蛋白超家族。LHC基因是核编码的，而色素-蛋白复合体定位于类囊体膜上，为跟踪不同色素沉着的质体的进化路径提供了一个标记。LHC分为绿藻、真球藻和高等植物的叶绿素a/b结合蛋白和不同藻类的叶绿素a/b结合蛋白。这项工作从另外三个分类群中研究了LHC的系统发育地位：视芽植物、隐芽植物和绿藻。对LHC序列的系统发育分析为叶绿体a/c结合蛋白谱系中的视藻和隐藻LHC序列的聚类提供了强有力的统计支持，该谱系包括异鞭藻的岩藻黄质-叶绿素蛋白(FCP)和甲藻的固有膜蛋白-叶绿素蛋白(IPCP)，这些关联表明来自异鞭藻、半裸藻、隐藻和甲藻的质体是从类似红藻的祖先进化而来的。Cllorarachnion LHC是叶绿素a/b结合蛋白组合的一部分，与色素沉着一致，进一步证明了其叶绿体是从绿藻次生内共生进化而来的。Cllorarachnion LHC序列与绿藻LHC聚在一起，后者主要与光系统II(LHCII)有关。这表明绿藻内共生体是在不同的LHCI和LHCII复合体出现后获得的。
The light-harvesting complexes (LHCs) are a superfamily of chlorophyll-binding proteins present in all photosynthetic eukaryotes. The Lhc genes are nuclear-encoded, yet the pigment-protein complexes are localized to the thylakoid membrane and provide a marker to follow the evolutionary paths of plastids with different pigmentation. The LHCs are divided into the chlorophyll a/b-binding proteins of the green algae, euglenoids, and higher plants and the chlorophyll a/b-binding proteins of various algal taxa. This work examines the phylogenetic position of the LHCs from three additional taxa: the rhodophytes, the cryptophytes, and the chlorarachniophytes. Phylogenetic analysis of the LHC sequences provides strong statistical support for the clustering of the rhodophyte and cryptomonad LHC sequences within the chlorophyll a/c-binding protein lineage, which includes the fucoxanthin-chlorophyll proteins (FCP) of the heterokonts and the intrinsic peridinin-chlorophyll proteins (iPCP) of the dinoflagellates, These associations suggest that plastids from the heterokonts, haptophytes, cryptomonads, and the dinoflagellate, Amphidinium, evolved from a red algal-like ancestor. The Chlorarachnion LHC is part of the chlorophyll a/b-binding protein assemblage, consistent with pigmentation, providing further evidence that its plastid evolved from a green algal secondary endosymbiosis. The Chlorarachnion LHC sequences cluster with the green algal LHCs that are predominantly associated with photosystem II (LHCII). This suggests that the green algal endosymbiont that evolved into the Chlorarachnion plastid was acquired following the emergence of distinct LHCI and LHCII complexes.