LARGE-SCALE METHYLATION PATTERNS IN THE NUCLEAR GENOMES OF PLANTS
LARGE-SCALE METHYLATION PATTERNS IN THE NUCLEAR GENOMES OF PLANTS
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DOI：
10.1016/0378-1119(92)90211-7
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发表时间：
1992-12-15
期刊：
影响因子：
3.5
通讯作者：
BERNARDI, G
中科院分区：
文献类型：
--
作者：
MATASSI, G;MELIS, R;BERNARDI, G
对来自五种植物(洋葱、玉米、黑麦、豌豆和烟草)的核DNA制剂(大小为50-100kb)的组成部分进行了甲基化研究，发现甲基化程度从GC贫组分增加到GC丰富组分。这种甲基化梯度在不同的植物中表现出不同的模式，因此出现了。为了代表一种新的、具有特征的基因组特征，它涉及到非编码的基因间序列，这些序列构成了所研究的大部分植物基因组，主要由重复序列组成。讨论了这些结果的结构和功能含义。
Methylation was investigated in compositional fractions of nuclear DNA preparations (50-100 kb in size)from five plants (onion, maize, rye, pea and tobacco), and was found to increase from GC-poor to GC-rich fractions. This methylation gradient showed different patterns in different plants and appears, therefore. to represent a novel, characteristic genome feature which concerns the noncoding, intergenic sequences that make up the bulk of the plant genomes investigated and mainly consist of repetitive sequences. The structural and functional implications of these results are discussed.