Impaired hair placode formation with reduced expression of hair follicle-related genes in mice lacking Lgr4
Impaired hair placode formation with reduced expression of hair follicle-related genes in mice lacking Lgr4
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DOI：
10.1002/dvdy.21639
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发表时间：
2008-08-01
影响因子：
2.5
通讯作者：
Nishimori, Katsuhiko
中科院分区：
文献类型：
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作者：
Mohri, Yasuaki;Kato, Shigeki;Nishimori, Katsuhiko
我们观察到Lgr 4（K5）（KO）小鼠具有稀疏的头发和耳后的局灶性脱发，如在虎斑（Eda）和无绒毛（Edar）小鼠中所观察到的，这是人类遗传性疾病少汗（无汗）外胚层发育不良（HED）的模型。Lgr 4缺陷小鼠表现出部分毛囊发育障碍，表皮中对毛囊形态发生至关重要的Edar、Lef 1和Shh表达减少。使用shh抗体对Lgr 4（-/-）小鼠表皮进行的免疫组织化学分析显示毛板数量减少，并且我们还检测到Smad（1/5/8）的更高磷酸化，这是抑制正常毛囊诱导所必需的。我们推测Lgr 4可能是一个新的调控毛囊发育的基因。
We observed that Lgr4(K5) (KO) mice had sparse head hair and focal alopecia behind their ears, as observed in tabby (Eda) and downless (Edar) mice, which are models of the human genetic disorder hypohidrotic (anhidrotic) ectodermal dysplasia (HED). Lgr4-deficient mice showed partial impairment in hair follicle development with reduced expression of Edar, Lef1, and Shh, which were essential for hair follicle morphogenesis, in the epidermis. Immunohistochemical analysis of Lgr4(-/-) mice epidermis using shh antibody showed reduced numbers of hair placodes, and we also detected higher phosphorylation of Smad(1/5/8), which is required to suppress normal hair follicle induction. We suspected that Lgr4 might be a novel gene class regulating the development of hair follicles.