Measurement of glycated hemoglobin (HbA1c) with an automated POCT instrument in comparison with HPLC and automated immunochemistry method:: evaluation of the influence of hemoglobin variants
Measurement of glycated hemoglobin (HbA1c) with an automated POCT instrument in comparison with HPLC and automated immunochemistry method:: evaluation of the influence of hemoglobin variants
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DOI：
10.1515/cclm.2006.041
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发表时间：
2006-02-01
影响因子：
6.8
通讯作者：
Makris, K
中科院分区：
文献类型：
--
作者：
Haliassos, A;Drakopoulos, I;Makris, K
我们的研究评估了一种快速简便的方法来进行即时检测（POCT）糖化血红蛋白HbA（1c）的测量，并与自动生化分析仪和阳离子交换色谱法上的免疫测定法进行比较，这两种方法通常用于临床实验室测量HbA（1c）。我们研究的一个重要发现是，尽管POCT仪器说明书声称该方法不受杂合状态或与β-地中海贫血组合的HbS存在的影响，但在某些血红蛋白病病例中发现了不一致的结果。在这些情况下，两种POCT和实验室免疫测定方法对含有HbS性状的样本显示出临床显著的阳性干扰。我们的结论是，使用POCT仪器进行测试时，应谨慎解读具有HbS特征的样本。
Our study evaluates a fast and easy way to perform point-of-care testing (POCT) measurements of glycated hemoglobin HbA(1c) in comparison with an immunoassay on an automated biochemistry analyzer and cation exchange chromatography, the two methods routinely used in clinical laboratories for the measurement of HbA(1c). A significant finding of our study is that although the POCT instrument insert claims that the method is not affected by the presence of HbS in the heterozygous state or in combination with beta-thalassemia, discrepant results were found in some cases with such hemoglobinopathies. In these cases, the two POCT and laboratory immunoassay methods showed clinically significant positive interferences with samples containing the HbS trait. We conclude that samples with the HbS trait should be interpreted with caution when tested using the POCT instrument.