IDENTIFICATION AND PREDICTED SEQUENCE OF A PREVIOUSLY UNRECOGNIZED SMALL HYDROPHOBIC PROTEIN, SH, OF THE PARAMYXOVIRUS SIMIAN VIRUS-5
IDENTIFICATION AND PREDICTED SEQUENCE OF A PREVIOUSLY UNRECOGNIZED SMALL HYDROPHOBIC PROTEIN, SH, OF THE PARAMYXOVIRUS SIMIAN VIRUS-5
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DOI：
10.1128/jvi.55.3.744-751.1985
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发表时间：
1985-01-01
影响因子：
5.4
通讯作者：
LAMB, RA
中科院分区：
文献类型：
--
作者：
HIEBERT, SW;PATERSON, RG;LAMB, RA
在副粘病毒猴病毒5型的病毒粒子RNA上，在融合蛋白基因和血凝素神经氨酸酶基因之间发现了一个未知基因(SH)。从SH基因转录得到一个含有292个核苷酸(加上多聚腺苷残基)的SH mRNA。SH基因含有一个开放阅读框，编码44个氨基酸组成的多肽，相对分子质量为5012。SH多肽被预测包含一个广泛的疏水区。在猴病毒5感染的细胞中检测到该蛋白，并通过体外翻译、杂交抑制翻译和杂交选择翻译证明该蛋白是由SH基因编码的。
A previously unrecognized gene (SH) was identified on the virion RNA of the paramyxovirus simian virus 5 between the genes for the fusion protein and the hemagglutinin-neuraminidase. An SH mRNA of 292 nucleotides (plus polyadenylate residues), transcribed from the SH gene, was identified. The SH mRNA contains a single open reading frame which encodes a polypeptide of 44 amino acids with a MW of 5012. The SH polypeptide is predicted to contain an extensive hydrophobic region. This protein was identified in simian virus 5-infected cells, and it was shown to be encoded by the SH mRNA by in vitro translation of size-fractionated mRNA species, hybrid-arrest translation and hybrid-selection translation.