A live attenuated severe acute respiratory syndrome coronavirus is immunogenic and efficacious in Golden Syrian hamsters
A live attenuated severe acute respiratory syndrome coronavirus is immunogenic and efficacious in Golden Syrian hamsters
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DOI：
10.1128/jvi.00304-08
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发表时间：
2008-08-01
影响因子：
5.4
通讯作者：
Subbarao, Kanta
中科院分区：
文献类型：
--
作者：
Lamirande, Elaine W.;DeDiego, Marta L.;Subbarao, Kanta
用仓鼠研究了由缺失E基因的重组SARS冠状病毒（rSARS-CoV-DeltaE）组成的减毒活疫苗的免疫原性和保护效力。用rSARS-CoV-Delta E免疫的仓鼠产生了高血清中和抗体滴度，并保护其免受同源（SARS-CoV Urbani）和异源（GD 03）SARS-CoV在上呼吸道和下呼吸道中的复制。rSARS-CoV-Delta E免疫的仓鼠在野生型病毒攻击后保持活性，而模拟免疫的仓鼠显示活性降低。尽管rSARS-CoV-Delta E在呼吸道中的复制能力减弱，但它在仓鼠中仍是一种具有免疫原性且有效的疫苗。
The immunogenicity and protective efficacy of a live attenuated vaccine consisting of a recombinant severe acute respiratory syndrome (SARS) coronavirus lacking the E gene (rSARS-CoV-Delta E) were studied using hamsters. Hamsters immunized with rSARS-CoV-Delta E developed high serum-neutralizing antibody titers and were protected from replication of homologous (SARS-CoV Urbani) and heterologous (GD03) SARS-CoV in the upper and lower respiratory tract. rSARS-CoV-Delta E-immunized hamsters remained active following wildtype virus challenge, while mock-immunized hamsters displayed decreased activity. Despite being attenuated in replication in the respiratory tract, rSARS-CoV-Delta E is an immunogenic and efficacious vaccine in hamsters.