DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis
DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis
复制标题
DOI：
10.1016/s0092-8674(00)80197-x
复制
发表时间：
1997-04-18
期刊：
影响因子：
64.5
通讯作者：
Wang, XD
中科院分区：
文献类型：
--
作者：
Liu, XS;Zou, H;Wang, XD
我们从 HeLa 细胞质中鉴定并纯化了一种蛋白质，该蛋白质被 caspase-3 激活后，会在共孵育细胞核中诱导 DNA 片段化。这种蛋白质被称为 DNA 片段因子 (DFF)，是 40 kDa 和 45 kDa 亚基的异二聚体。从其 cDNA 序列确定的 45 kDa 亚基的氨基酸序列表明它是一种新型蛋白质。 Caspase-3 在两个位点切割 45 kDa 亚基，产生活性因子，该因子可产生 DNA 片段化，而无需进一步需要 caspase-3 或其他胞质蛋白。在经历凋亡的细胞中，45 kDa 亚基的切割模式与体外 caspase-3 的切割模式相同。这些数据描绘了细胞凋亡过程中的直接信号转导途径：caspase-3 到 DFF 到 DNA 片段化。
We have identified and purified from HeLa cytosol a protein that induces DNA fragmentation in coincubated nuclei after it is activated by caspase-3. This protein, designated DNA Fragmentation Factor (DFF), is a heterodimer of 40 kDa and 45 kDa subunits. The amino acid sequence of the 45 kDa subunit, determined from its cDNA sequence, reveals it to be a novel protein. Caspase-3 cleaves the 45 kDa subunit at two sites to generate an active factor that produces DNA fragmentation without further requirement for caspase-3 or other cytosolic proteins. In cells undergoing apoptosis, the 45 kDa subunit is cleaved in the same pattern as it is cleaved by caspase-3 in vitro. These data delineate a direct signal transduction pathway during apoptosis: caspase-3 to DFF to DNA fragmentation.