ANTIGEN PRESENTATION ENHANCED BY THE ALTERNATIVELY SPLICED INVARIANT CHAIN GENE-PRODUCT P41
ANTIGEN PRESENTATION ENHANCED BY THE ALTERNATIVELY SPLICED INVARIANT CHAIN GENE-PRODUCT P41
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DOI：
10.1038/357596a0
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发表时间：
1992-06-18
期刊：
影响因子：
64.8
通讯作者：
MILLER, J
中科院分区：
文献类型：
--
作者：
PETERSON, M;MILLER, J
在生物合成过程中，主要组织相容性复合体的II类分子与一种称为不变链II的非多态性蛋白相关，该蛋白促进II类分子的折叠并使其从内质网1-4中退出，干扰其与肽5,6的关联并指导其后高尔基转运（参考文献7-9）。如果Ii阻断内质网中装载内源抗原的Ii类分子和/或将Ii类分子引导到外源抗原装载室，那么Ii类分子的共表达应该增强Ii类分子向T细胞呈递外源抗原的能力。但是支持Ii在Ii类限制性抗原呈递中的作用的数据是有争议的1,10-13。在这里，我们表明Ii可以促进抗原子集的外源抗原呈递。虽然li的所有已知功能都归因于Ii的主要形式，p31，但我们发现在大多数情况下，抗原呈递仅由Ii的次要形式，p41进行选择性剪接。
DURING biosynthesis, class II molecules of the major histocompatibility complex are associated with a nonpolymorphic protein called invariant chain, Ii, which facilitates folding of class II molecules and their exit from the endoplasmic reticulum 1-4, interferes with their association with peptide 5,6 and directs their post-Golgi transport (refs 7-9). If Ii blocks class II loading with endogenous antigens in the endoplasmic reticulum and/or directs class II molecules to the exogenous antigen-loading compartment, then the co-expression of Ii should enhance the ability of class II molecules to present exogenous antigens to T cells. But data supporting a role for Ii in class II-restricted antigen presentation are controversial 1,10-13. Here we show that Ii can facilitate exogenous antigen presentation for a subset of antigens. Although all known functions of li have been ascribed to the principal form of Ii, p31, we find that in most cases antigen presentation is facilitated only by the alternatively spliced, minor form of Ii, p41.