Core histones of the amitochondriate protist, Giardia lamblia.
Core histones of the amitochondriate protist, Giardia lamblia.
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无线粒体原生生物兰氏贾第鞭毛虫的核心组蛋白。
DOI：
10.1093/oxfordjournals.molbev.a026398
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发表时间：
2000
影响因子：
10.7
通讯作者：
Mllerm,M
中科院分区：
文献类型：
--
作者：
Wu,G;McArthur,AG;Fiser,A;Sali,A;Sogin,ML;Mllerm,M
对兰氏贾第鞭毛虫核心组蛋白 H2a、H2b、H3 和 H4 的编码基因进行了测序。在所有核心组蛋白基因的编码区上游发现了一个保守的生物体和基因特异性元件 GRGCGCAGATTTVGG。所有四种组蛋白的衍生氨基酸序列与其他真核生物中的同源物相似，尽管它们是该蛋白质家族中差异最大的成员之一。比较蛋白质结构建模与所得模型的能量评估相结合表明，theG. lamblacore组蛋白单独和一起可以呈现与通过X射线晶体学为非洲爪蟾和核小体核心颗粒建立的相同的三维结构。自从G. lamlia 代表了许多不同分子树中最早分化的真核生物之一，其组蛋白的结构可能与理解组蛋白进化相关。 TheG.兰伯利亚蛋白不代表古菌组蛋白和真核组蛋白之间的中间阶段。
Genes coding for the core histones H2a, H2b, H3, and H4 ofGiardia lambliawere sequenced. A conserved organism- and gene-specific element, GRGCGCAGATTTVGG, was found upstream of the coding region in all core histone genes. The derived amino acid sequences of all four histones were similar to their homologs in other eukaryotes, although they were among the most divergent members of this protein family. Comparative protein structure modeling combined with energy evaluation of the resulting models indicated that theG. lambliacore histones individually and together can assume the same three-dimensional structures that were established by X-ray crystallography forXenopus laevishistones and the nucleosome core particle. SinceG. lambliarepresents one of the earliest-diverging eukaryotes in many different molecular trees, the structure of its histones is potentially of relevance to understanding histone evolution. TheG. lambliaproteins do not represent an intermediate stage between archaeal and eukaryotic histones.