Biogenic synthesis of silver nanoparticles and their synergistic effect with antibiotics: a study against gram-positive and gram-negative bacteria
Biogenic synthesis of silver nanoparticles and their synergistic effect with antibiotics: a study against gram-positive and gram-negative bacteria
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DOI：
10.1016/j.nano.2009.04.006
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发表时间：
2010-02-01
影响因子：
5.4
通讯作者：
Venketesan, Ramasamy
中科院分区：
文献类型：
--
作者：
Fayaz, Amanulla Mohammed;Balaji, Kulandaivelu;Venketesan, Ramasamy
开发一种可靠的绿色化学方法来生物合成纳米材料是当前纳米技术研究的一个重要方面。银纳米颗粒（AgNPs）因其抑制和杀菌作用而被人们所熟知。近年来，病原菌对抗菌剂的耐药性已经出现，这是医疗保健行业面临的一个重大挑战。在本研究中，报道了绿色木霉用于从银银溶液中胞外生物合成AgNPs的用途。结果表明，当接触T.绿色，在溶液中被还原，从而导致形成非常稳定的AgNP。这些银纳米粒子的特点是通过几种技术。在紫外-可见光谱中，纳米粒子在420 nm处有最大吸收。通过傅立叶变换红外光谱法鉴定蛋白质的存在。Ag+离子还原为元素银的特征在于通过X-射线光电子分光光度法。评价AgNP在1 × 10（-3）mol dm（-3）水溶液中作为pH的函数的电动力学测量（zeta电位）。透射电子显微照片揭示了5-40 nm的多分散纳米颗粒的形成，并且通过能量分散光谱分析证实了元素银的存在。还评估了纳米颗粒与各种抗生素对革兰氏阳性和革兰氏阴性细菌的增加的抗微生物活性。氨苄青霉素，卡那霉素，红霉素，氯霉素的抗菌活性增加的AgNPs对测试菌株的存在。观察到氨苄青霉素对测试菌株的最高增强效果。结果表明，抗菌素与纳米银复合后具有较好的抗菌效果。临床编辑认为：银纳米颗粒（Ag NP-s）代表了在对抗多耐药细菌方面的一个重要的纳米医学进展。在这项研究中，绿色木霉用于胞外生物合成非常稳定的Ag Nps。在纳米银的存在下，对测试菌株的卡那霉素，红霉素，氯霉素，特别是氨苄青霉素的抗菌活性增加。(C)2010年爱思唯尔公司All rights reserved.
The development of a reliable green chemistry process for the biogenic synthesis of nanomaterials is an important aspect of current nanotechnology research. Silver nanoparticles (AgNPs) have been known for their inhibitory and bactericidal effect. Resistance to antimicrobial agents by pathogenic bacteria has emerged in recent years and is a major challenge for the health care industry. In the present investigation the use of the fungus Trichoderma viride for the extracellular biosynthesis of AgNPs from silver nitrate solution is reported. It was observed that the aqueous silver (Ag+) ions, when exposed to a filtrate of T. viride, were reduced in solution, thereby leading to formation of extremely stable AgNPs. These AgNPs were characterized by means of several techniques. The nanoparticles show maximum absorbance at 420 nm on ultraviolet-visible spectra. The presence of proteins was identified by Fourier transform-infrared spectroscopy. The reduction of Ag+ ions to elemental silver was characterized by x-ray photoelectron spectrophotometry. Electrokinetic measurements (zeta potential) of AgNPs as a function of pH in 1 x 10(-3) mol dm(-3) aqueous solution were evaluated. The transmission electron micrograph revealed the formation of polydispersed nanoparticles of 5-40 nm, and the presence of elemental silver was confirmed by energy-dispersed spectroscopy analysis. The nanoparticles were also evaluated for their increased antimicrobial activities with various antibiotics against gram-positive and gram-negative bacteria. The antibacterial activities of ampicillin, kanamycin, erythromycin, and chloramphenicol were increased in the presence of AgNPs against test strains. The highest enhancing effect was observed for ampicillin against test strains. The result showed that the combination of antibiotics with AgNPs have better antimicrobial effects. A mechanism was also proposed to explain this phenomenon.From the Clinical Editor: Silver nanoparticles (Ag NP-s) represent an important nanomedicine-based advance in the fight against polyresistent bacteria. In this study, the fungus Trichoderma viride was utilized for extracellular biosynthesis of extremely stable Ag Nps. The antibacterial activities of kanamycin, erythromycin, chloramphenicol and especially of ampicillin were increased in the presence of Ag NPs against test strains. (C) 2010 Elsevier Inc. All rights reserved.