Real-time analysis of living animals by electrospray ionization mass spectrometry.
Real-time analysis of living animals by electrospray ionization mass spectrometry.
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DOI：
10.1016/j.ab.2011.06.020
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发表时间：
2011-10
影响因子：
2.9
通讯作者：
K. Yoshimura;L. Chen;Zhan Yu;K. Hiraoka;S. Takeda
中科院分区：
文献类型：
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作者：
K. Yoshimura;L. Chen;Zhan Yu;K. Hiraoka;S. Takeda
探针电喷雾电离（PESI）是生化分析中最有前途的方法之一，因为它使我们能够非常快速地分析生物样品，而无需任何特殊的预处理。此外，由于针尖尺寸较小，该方法具有对样品侵入性低等优点，使得原位分析活体动物器官或组织的生物学特征成为可能。在这项研究中，我们对活体小鼠进行了实时分析，描绘了正常小鼠和脂肪变性小鼠之间肝细胞脂质组成的差异。在脂肪变性小鼠中，三酰甘油的峰数和离子丰度比对照小鼠高得多。本研究中使用的所有小鼠都很好地耐受了该过程，并存活了一个多月，直到被牺牲以进行进一步分析。为了测试医学诊断的潜力，还测量了人类肿瘤组织，我们获得了被认为对诊断有用的判别结果。这些结果为 PESI 在生物分子体内分析中的应用铺平了道路。
Probe electrospray ionization (PESI) is one of the most promising methods in biochemical analysis because it enables us to analyze biological samples very quickly without any special pretreatment. Moreover, due to the small size of the needle tip, this method has advantages such as low invasiveness to the samples, making it possible to analyze the biological profiles of organs or tissues in living animal in situ. In this study, we performed a real-time analysis of living mice that delineates the differences in lipid composition of hepatocytes between normal and steatotic mice. In steatotic mice, the number of peaks and the ion abundance for triacylglycerols were much higher compared with those of control mice. All mice used in this study tolerated the procedure well and survived for more than a month until sacrificed for further analysis. To test a potential for medical diagnosis, human tumor tissues were also measured and we obtained discriminative results judged as useful for diagnostics. These results pave the way into the application of PESI to the in vivo analysis of biological molecules.