Data-deposition protocols for correlative soft X-ray tomography and super-resolution structured illumination microscopy applications.

Data-deposition protocols for correlative soft X-ray tomography and super-resolution structured illumination microscopy applications.
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DOI:
10.1016/j.xpro.2020.100253
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发表时间:
2021-03-19
期刊:
影响因子:
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通讯作者:
Kleywegt GJ
Kleywegt GJ
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其他
文献类型:
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作者:
Iudin A;Sarkans U;Koronfel MA;Fish T;Dobbie I;Harkiolaki M;Patwardhan A;Kleywegt GJ

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This protocol illustrates the steps necessary to deposit correlated 3D cryo-imaging data from cryo-structured illumination microscopy and cryo-soft X-ray tomography with the BioStudies and EMPIAR deposition databases of the European Bioinformatics Institute. There is currently a real need for a robust method of data deposition to ensure unhindered access to and independent validation of correlative light and X-ray microscopy data to allow use in further comparative studies, educational activities, and data mining. For complete details on the use and execution of this protocol, please refer to. Step-by-step guide for CLXM data deposition to EMBL-EBI archives Establishes technical metadata requirements for efficient image sharing and reuse Example deposition of CLXM dataset from a study of reovirus infection in U2OS cells This protocol illustrates the steps necessary to deposit correlated 3D cryo-imaging data from cryo-structured illumination microscopy and cryo-soft X-ray tomography with the BioStudies and EMPIAR deposition databases of the European Bioinformatics Institute. There is currently a real need for a robust method of data deposition to ensure unhindered access to and independent validation of correlative light and X-ray microscopy data to allow use in further comparative studies, educational activities, and data mining.
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