Chronic ether stress-induced response of urocortin 1 neurons in the Edinger-Westphal nucleus in the mouse
Chronic ether stress-induced response of urocortin 1 neurons in the Edinger-Westphal nucleus in the mouse
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DOI：
10.1016/j.brainres.2005.04.012
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发表时间：
2005-06-07
期刊：
影响因子：
2.9
通讯作者：
Kozicz, T
中科院分区：
文献类型：
--
作者：
Korosi, A;Schotanus, S;Kozicz, T
尿皮质素 1 (Ucn1) 神经元在 Edinger-Westphal 核 (E-WN) 中表达最丰富，可对各种急性挑战做出反应。在最近的一项研究中，我们发现急性乙醚应激导致 E-WN Ucn1 细胞最强的激活，正如 Fos 免疫组织化学（通常用作神经元激活标记）所揭示的那样。尽管 E-WN Ucn1 神经元的急性应激反应已被广泛研究，但这些神经元中 Fos 响应重复挑战的激活模式尚未得到研究。因此，我们定量研究了小鼠急性和慢性乙醚应激后 E-WN 神经元中 Fos 的激活，并测量了 E-WN 神经元中 Ucn1 mRNA 的水平。与非应激小鼠相比，急性应激导致 Fos 反应强烈，并且 Ucn1 mRNA 增加。在慢性应激范例中，Fos 表达没有变化，而每日乙醚暴露 2 周和 3 周后，E-INN 中 Ucn1 mRNA 表达急剧下降。 Fos 和 Ucn1 mRNA 在急性和慢性应激动物的 E-WN 神经元中共表达。本文首次证明 E-WN 中表达 Ucn1 mRNA 的神经元对慢性同型乙醚挑战表现出非习惯性 Fos 反应，与急性应激动物相比，这也导致 E-WN Ucn1 mRNA 水平可靠的下调。基于这些结果，我们提出E-WN-Ucn1系统代表了一种新的应激适应途径，可能在应对长期挑战中发挥重要作用。 (c) 2005 Elsevier B.V. 保留所有权利。
Urocortin 1 (Ucn1) neurons, most abundantly expressed in the Edinger-Westphal nucleus (E-WN), respond to various acute challenges. In a recent study, we found that acute ether stress resulted in the strongest activation of E-WN Ucn1 cells, as revealed by immunohistochemistry for Fos (often used as a marker for neuronal activation). Although the acute stress responsiveness of E-WN Ucn1 neurons has been widely studied, the activation pattern of Fos in these neurons in response to repeated challenges has not yet been investigated. Therefore, we quantitatively studied Fos activation in E-WN neurons and measured Ucn1 mRNA levels in E-WN neurons after acute and chronic ether stress in mice. Acute stress resulted in a robust Fos response and an increase in Ucn1 mRNA as compared to non-stressed mice. In the chronic stress paradigm, Fos expression was unchanged, whereas after 2 and 3 weeks of daily ether exposure Ucn1 mRNA expression had strongly declined in the E-INN. Fos and Ucn1 mRNA were co-expressed in E-WN neurons in both acutely and chronically stressed animals.This paper is the first to demonstrate that Ucn1 mRNA-expressing neurons in the E-WN show a non-habituating Fos response to a chronic homotypic ether challenge that also resulted in a reliable down-regulation of E-WN Ucn1 mRNA levels vs. acutely stressed animals. Based on these results, we propose that the E-WN-Ucn1 system represents a novel stress adaptation pathway, which may play an important role in coping with chronic challenges. (c) 2005 Elsevier B.V. All rights reserved.