β2‐glycoprotein I (β2‐GPI) mRNA is expressed by several cell types involved in anti‐phospholipid syndrome‐related tissue damage
β2‐glycoprotein I (β2‐GPI) mRNA is expressed by several cell types involved in anti‐phospholipid syndrome‐related tissue damage
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β2-糖蛋白 I (β2-GPI) mRNA 由参与抗磷脂综合征相关组织损伤的几种细胞类型表达
DOI：
10.1046/j.1365-2249.1999.00770.x
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发表时间：
1999
影响因子：
4.6
通讯作者：
Valesini
中科院分区：
文献类型：
--
作者：
Caronti;Calderaro;Alessandrì;Conti;Tinghino;Palladini;Valesini
我们在此报告了参与抗磷脂综合征（APS）病理生理学的细胞类型的β2-GPI mRNA表达，即作为APS中自身抗体靶点的内皮细胞、参与中枢神经系统（CNS）APS的星形胶质细胞和神经元。本研究还纳入了淋巴细胞，因为已证明系统性红斑狼疮相关CNS疾病患者的血清抗淋巴细胞抗体与脑抗原交叉反应，鞘内合成抗神经元抗体。逆转录酶-聚合酶链反应，然后对所得产物进行限制性酶消化，证明在存在和不存在胎牛血清的情况下培养的所有供试细胞类型中均存在β2-GPI mRNA。在两种培养条件下，通过间接免疫荧光技术测定，相同细胞类型对抗β2-GPI单克隆抗体具有免疫反应性。总之，这些结果表明β2-GPI的直接细胞合成，表明β2-GPI在APS中的抗原功能，包括该综合征中发生的CNS疾病。
We report here the expression of β2‐GPI mRNA by cell types involved in the pathophysiology of the anti‐phospholipid syndrome (APS), i.e. endothelial cells as a target of autoantibodies in the APS, astrocytes and neurones involved in APS of the central nervous system (CNS). Lymphocytes were also included in the study, as it has been demonstrated that patients with systemic lupus erythematosus‐associated CNS diseases have serum anti‐lymphocyte antibodies cross‐reacting with brain antigens, and intrathecally synthesized anti‐neurone antibodies. Reverse transcriptase‐polymerase chain reaction followed by restriction enzyme digestion of the product obtained demonstrated the presence of β2‐GPI mRNA in all cell types here tested, cultured both in presence and absence of fetal calf serum. In both culture conditions, the same cell types were immunoreactive to an anti‐β2‐GPI MoAb, as determined by indirect immunofluorescence technique. Taken together, these results indicate a direct cell synthesis of β2‐GPI, suggesting an antigenic function of β2‐GPI in the APS, including the CNS disease that occurs in this syndrome.
抗磷脂综合征患者是否有 β2-糖蛋白 I 自身抗体？
DOI：
--
发表时间：
1993
期刊：
The Journal of laboratory and clinical medicine
影响因子：
--
作者：
Gharavi,AE;Harris,EN;Sammaritano,LR;Pierangeli,SS;Wen,J
通讯作者：
Wen,J
与抗磷脂抗体相关的一系列神经系统疾病。
影响因子：
--
作者：
Levine,SR;Welch,KM
通讯作者：
Welch,KM
抗磷脂综合征患者胎盘中的 Beta 2 糖蛋白 I 和胎盘抗凝蛋白 I。
DOI：
--
发表时间：
1994
期刊：
The Journal of rheumatology
影响因子：
--
作者：
LaRosa,L;Meroni,PL;Tincani,A;Balestrieri,G;Faden,D;Lojacono,A;Morassi,L;Brocchi,E;DelPapa,N;Gharavi,A
通讯作者：
Gharavi,A