MAXIMUM FLUID CONCENTRATIONS OF MATERIALS RELEASED FROM PLATELETS AT A SURFACE
MAXIMUM FLUID CONCENTRATIONS OF MATERIALS RELEASED FROM PLATELETS AT A SURFACE
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DOI：
10.1152/ajpheart.1983.244.1.h109
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发表时间：
1983-01-01
影响因子：
--
通讯作者：
FEUERSTEIN, IA
中科院分区：
文献类型：
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作者：
ADAMS, GA;FEUERSTEIN, IA
检查了在血小板在胶原涂层玻璃上积聚期间从[人]血小板的致密颗粒和α-颗粒释放的物质的局部浓度的估计。通过内径 1.3 毫米的管灌注血小板/红细胞悬浮液。基于扩散和对流的计算程序中使用了经验数据，旨在得出每种所考虑物质的界面流体浓度 (IFC) 的上限。必要的经验数据是血小板积累的速率和血小板中能够分泌的物质的最大量。 IFC 取决于表面 (G) 的剪切速率，并与 G0.27 成正比。这意味着流量增加 8 倍将增加 IFC .apprx。 2倍。血清素、焦磷酸盐、AMP 和 ATP 的含量不足以产生 IFC，如果在 IFC 中单独使用，IFC 可能会影响血小板聚集蛋白。第二组材料，纤维蛋白原、纤连蛋白血管性血友病因子和 Ca，其 IFC 浓度低于血浆中正常浓度。第三类仅含有 ADP，其 IFC 与已知影响血小板聚集的 IFC 接近。花生四烯酸代谢物可能促进或抑制血小板聚集，其作用有待进一步描述。
The estimation of local concentrations of materials that are released from the dense and .alpha.-granules of [human] platelets during accumulation of platelets on collagen-coated glass was examined. Platelet/red blood cell suspensions were perfused through a 1.3-mm-ID [inside diameter] tube. Empirical data were used in a calculation procedure, based on diffusion and convection, designed to yield an upper bound on the interfacial fluid concentration (IFC) for each substance considered. The necessary empirical data are the rate of platelet accumulation and the maximum amount of material in the platelet capable of secretion. The IFC is dependent on the shear rate at the surface (G) and is proportional to G0.27. This means that an 8-fold increase in flow rate would increase the IFC .apprx. 2-fold. Serotonin, pyrophosphate, AMP and ATP were not present in sufficient quantities to produce IFC that could influence platelet aggregatin if used alone at the IFC. A 2nd set of materials, fibrinogen, fibronectin von Willebrand factor and Ca, had IFC less than their concentrations normally found in plasma. A 3rd category, containing ADP alone, had an IFC close to those known to affect platelet aggregation. The role of metabolites of arachidonic acid, which may promote or inhibit platelet aggregation, awaits further description.