INVIVO SE-75 BINDING TO HUMAN PLASMA-PROTEINS AFTER ADMINISTRATION OF SE-75(O2/3)
INVIVO SE-75 BINDING TO HUMAN PLASMA-PROTEINS AFTER ADMINISTRATION OF SE-75(O2/3)
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DOI：
10.1016/0304-4165(74)90188-3
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发表时间：
1974-01-01
期刊：
影响因子：
--
通讯作者：
BURK, RF
中科院分区：
文献类型：
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作者：
BURK, RF
在4名接受200-250 μCi 7 5SeO 32 −（1.25 μg硒）静脉注射进行肿瘤扫描的癌症患者中，研究了7 5Se与血浆蛋白的结合。在注射后1小时内，血浆中75 Se迅速消失。凝胶过滤层析和透析实验表明，注射后1 ~ 6 h，大量的75 Se返回血浆，与蛋白结合。注射后3 min，血浆中极低密度脂蛋白和低密度脂蛋白中的75 Se含量高达16%，而高密度脂蛋白中的75 Se含量极少。极低密度脂蛋白7 5 Se活性下降很快，而低密度脂蛋白7 5 Se活性下降较慢。对一名受试者的脂蛋白进行的带状超电泳研究显示，从极低密度脂蛋白峰到低密度脂蛋白峰，有7种5Se结合蛋白连续存在。用8 M尿素变性或用0.5M巯基乙醇处理可除去大部分的7 - 5Se。这些治疗方法从注射后48小时收集的血浆中去除了很少的7 - 5硒，表明硒在脂蛋白中的结合类型与其他血浆蛋白不同。
Binding of7 5Se to plasma proteins was studied in four cancer patients who received 200–250 μCi of7 5SeO32−(1.25 μg of selenium) intravenously for tumor scans. During the hour after injection, the7 5Se disappeared rapidly from the plasma. Gel filtration chromatography and dialysis experiments indicated that a large amount of the7 5Se returned to the plasma bound to protein between 1 and 6 h after injection. Up to 16% of the plasma7 5Se was found in very-low-density lipoproteins and low-density lipoproteins as early as 3 mikn after injection but very little7 5Se was found in high density lipoproteins. The very low density lipoprotein7 5Se activity declined very rapidly whereas low density lipoprotein7 5Se activity fell more slowly. Zonal ultracentrifugal studies of one subject's lipoproteins revealed a continuum of7 5Se-binding proteins from the very low density lipoprotein peak to the low density lipoprotein peak. Most of the7 5Se could be removed from the lipoproteins by denaturation with 8 M urea or treatment with 0.5 M mercaptoethanol. These treatments removed very little of the7 5Se from plasma collected 48 h after injection indicating a different type of binding of selenium in lipoproteins than in other plasma proteins.