The effect of prenatal X-irradiation on the developing cerebral cortex of rats. II: A quantitative assessment of glial cells in the somatosensory cortex
The effect of prenatal X-irradiation on the developing cerebral cortex of rats. II: A quantitative assessment of glial cells in the somatosensory cortex
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DOI：
10.1016/j.ijdevneu.2007.05.003
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发表时间：
2007-08-01
影响因子：
1.8
通讯作者：
Takeuchi, Yoshiki
中科院分区：
文献类型：
--
作者：
Miki, Takanori;Yokoyama, Toshifumi;Takeuchi, Yoshiki
众所周知，发育中的中枢神经系统非常容易受到X射线的照射。虽然神经胶质细胞参与多种脑功能，但对X射线照射对神经胶质细胞的影响的了解有限。因此，本研究的目的是评估产前X射线照射对神经胶质细胞的影响。妊娠15天的Wistar大鼠接受1.0GyX射线照射。他们的后代在7周大时接受了检查。X射线照射组大鼠的前脑重量明显低于年龄匹配的对照组。体感皮质(SC)的组织学定量和体视学研究显示，X射线照射组和对照组大鼠的神经胶质细胞数密度无显著差异。然而，X射线照射的动物的神经胶质细胞的核大小明显较大。我们以前曾报道过，类似的X射线照射模式没有导致SC内神经元的数量密度发生显著变化。根据本研究的结果，受X射线照射的动物和对照动物之间的胶质细胞与神经元的比率没有显著差异。综上所述，推测产前X射线照射对神经胶质细胞和神经元的数密度具有相同的影响。(C)2007年综合业务数字网。爱思唯尔有限公司出版。保留所有权利。
The developing central nervous system is known to be highly vulnerable to X-irradiation. Although glial cells are involved in various brain functions, knowledge on the effects of X-irradiation on glial cells is limited. Therefore, the purpose of the present study was to evaluate the effects of prenatal X-irradiation on glial cells. Pregnant Wistar rats were exposed to X-irradiation at a dose of 1.0 Gy on day 15 of gestation. Their offspring were examined at 7 weeks of age. The forebrain weight of X-irradiated rats was significantly lower than that of the age-matched controls. Histological quantification with stereology of the somatosensory cortex (SC) revealed no significant difference in the numerical density of glial cells between the X-irradiated and control rats. However, the glial cells in the X-irradiated animals had significantly larger nuclear size. We had previously reported that a similar X-irradiation paradigm resulted in no significant change in the numerical density of neurons in the SC. According to the results of the present study, there were no significant differences in the glial cell-to-neuron ratios between the X-irradiated and control animals. Taken together, it is speculated that prenatal X-irradiation has an equal effects on the numerical densities of glial cells and neurons. (C) 2007 ISDN. Published by Elsevier Ltd. All rights reserved.