IONIC CONTROL OF LOCOMOTION AND SHAPE OF EPITHELIAL-CELLS .2. ROLE OF MONO-VALENT CATIONS
IONIC CONTROL OF LOCOMOTION AND SHAPE OF EPITHELIAL-CELLS .2. ROLE OF MONO-VALENT CATIONS
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DOI：
10.1002/cm.970100409
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发表时间：
1988-01-01
影响因子：
--
通讯作者：
VOTH, M
中科院分区：
文献类型：
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作者：
BEREITERHAHN, J;VOTH, M
本文研究了从爪蟾蝌蚪表皮分离的角膜基质细胞在体外的迁移。在盐水中，细胞以5-6 μ m/min的平均速度移动。在钠含量减少的盐水中，迁移减慢，在钠含量不超过4 mM的培养基中，迁移停止。piretanide抑制Na+/K+-2Cl-协同转运蛋白可使细胞迁移速度降低至对照水平的约三分之一，这与pH7.2时阿米洛利抑制Na+/H+反向转运蛋白的结果相同。然而，在pH 6.6时，阿米洛利仅轻微影响运动。细胞外K+浓度增加或哇巴因抑制Na+/K+泵引起的质膜去极化在超过1小时的过程中仅具有微小的影响。使用钠离子载体莫能菌素的细胞超极化阻碍运动;这种抑制依赖于活性Na+/K+泵。离子载体介导的K+梯度的破坏严格抑制运动。实验表明，钠离子的持续流动对于维持细胞运动是必不可少的。这些离子可能主要通过影响胞质游离钙浓度和pH值来发挥作用。
The migration of keratocytes isolated from Xenopus tadpole epidermis has been investigated in vitro. In saline the cells move with a mean speed of 5-6 .mu.m/min. Migration is slowed down in saline with diminished sodium content and ceases in media containing not more than 4 mM sodium. Inhibition of the Na+/K+-2Cl- cotransporter by piretanide reduces the speed of migrating cells to about one-third of the control level, the same accounts to inhibition of the Na+/H+ antiport with amiloride at pH 7.2. At pH 6.6, however, amiloride only slightly influences locomotion. Depolarization of the plasma membrane by increased extracellular K+ concentration or by inhibition of the Na+/K+ pump by ouabain is only of minor influence during more than 1 h. Hyperpolarization of the cells using the sodium ionophore monensin impedes locomotion; this inhibition depends on an active Na+/K+ pump. Ionophore-mediated breakdown of the K+ gradient strictly inhibits locomotion. The experiments have shown that a continuous flux of sodium ions is indispensable for the maintenance of cell locomotion. These ions may exert their action primarily by affecting cytosolic free calcium concentration and pH.