Raman microscopy for dynamic molecular imaging of living cells
Raman microscopy for dynamic molecular imaging of living cells
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DOI：
10.1117/1.2952192
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发表时间：
2008-07-01
影响因子：
3.5
通讯作者：
Kawata, Satoshi
中科院分区：
文献类型：
--
作者：
Hamada, Keisaku;Fujita, Katsumasa;Kawata, Satoshi
我们展示了动态成像的分子分布在未染色的活细胞使用拉曼散射。通过结合狭缝扫描检测和优化的激发波长，我们成像的动态分子分布的细胞色素c，蛋白质β片，和脂质在未染色的HeLa细胞的时间分辨率为3分钟。我们发现，532 nm激发可用于产生强拉曼散射信号，并抑制通常使拉曼信号模糊的自发荧光。利用这种技术，我们揭示了细胞色素c和其他生物分子在细胞质分裂过程中的时间分辨分布，而不需要荧光标记或标记。(C)2008年，由光学仪器工程师协会（Society of Photo-Optical Instrumentation Engineers）主办。
We demonstrate dynamic imaging of molecular distribution in unstained living cells using Raman scattering. By combining slit-scanning detection and optimizing the excitation wavelength, we imaged the dynamic molecular distributions of cytochrome c, protein beta sheets, and lipids in unstained HeLa cells with a temporal resolution of 3 minutes. We found that 532-nm excitation can be used to generate strong Raman scattering signals and to suppress autofluorescence that typically obscures Raman signals. With this technique, we reveal time-resolved distributions of cytochrome c and other biomolecules in living cells in the process of cytokinesis without the need for fluorescent labels or markers. (C) 2008 Society of Photo-Optical Instrumentation Engineers.