CLEAVAGE ORIENTATION AND THE ASYMMETRIC INHERITANCE OF NOTCH1 IMMUNOREACTIVITY IN MAMMALIAN NEUROGENESIS
CLEAVAGE ORIENTATION AND THE ASYMMETRIC INHERITANCE OF NOTCH1 IMMUNOREACTIVITY IN MAMMALIAN NEUROGENESIS
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DOI：
10.1016/0092-8674(95)90035-7
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发表时间：
1995-08-25
期刊：
影响因子：
64.5
通讯作者：
MCCONNELL, SK
中科院分区：
文献类型：
--
作者：
CHENN, A;MCCONNELL, SK
哺乳动物中枢神经系统中的神经元是由神经管腔附近的祖细胞产生的。在发生大脑皮层的切片中分裂细胞的延时显微镜表明，切割方向预示了子细胞的命运。垂直分裂在行为和形态上产生类似于前体细胞的女儿。这些对称分区可能有助于扩展或维护祖细胞池。相比之下，水平分裂的细胞产生的基础女儿的行为像年轻的迁徙神经元和顶端女儿一样，它们留在增殖区域内。 Notch1免疫反应性在有丝分裂细胞中不对称分布，Notch1由水平分裂的基底（神经元）女儿选择性地遗传。这些结果提供了细胞和分子证据，表明皮质神经元是由不对称分裂产生的。
Neurons in the mammalian central nervous system are generated from progenitor cells near the lumen of the neural tube. Time-lapse microscopy of dividing cells in slices of developing cerebral cortex reveals that cleavage orientation predicts the fates of daughter cells. Vertical cleavages produce behaviorally and morphologically identical daughters that resemble precursor cells; these symmetric divisions may serve to expand or maintain the progenitor pool. In contrast, horizontally dividing cells produce basal daughters that behave like young migratory neurons and apical daughters that remain within the proliferative zone. Notch1 immunoreactivity is distributed asymmetrically in mitotic cells, with Notch1 inherited selectively by the basal (neuronal) daughter of horizontal divisions. These results provide cellular and molecular evidence that cortical neurons are generated from asymmetric divisions.