A short G1 phase is an intrinsic determinant of naive embryonic stem cell pluripotency
A short G1 phase is an intrinsic determinant of naive embryonic stem cell pluripotency
复制标题
DOI：
10.1016/j.scr.2012.10.004
复制
发表时间：
2013-01-01
影响因子：
1.2
通讯作者：
Savatier, Pierre
中科院分区：
文献类型：
--
作者：
Coronado, Diana;Godet, Murielle;Savatier, Pierre
短 G1 期是小鼠胚胎干细胞 (ESC) 的一个特征。为了确定 G1 期限制与多能性之间是否存在因果关系，我们利用荧光泛素化细胞周期指示器 (FUCCI) 报告系统对不同细胞周期阶段的 ESC 进行 FACS 分类。因此，G1期细胞似乎更容易分化，特别是当ESC在多能性的初始状态下自我更新时。从基础到幼稚，然后从幼稚到启动的多能性状态的转变与 G1 期持续时间的增加有关，并且细胞周期蛋白 E 介导的 G1 IS 转变的改变改变了自我更新和分化之间的平衡。 LIE 的退出导致幼稚 ESC 中 G1 期的延长，这种延长发生在早期谱系特异性标记出现之前，并且可以在补充 LIE 后逆转。我们得出的结论是，在小鼠 ESC 中观察到的短 G1 期是幼稚多能性的决定因素，并且部分受 LIE 信号控制。 (C) 2012 Elsevier B.V. 保留所有权利。
A short G1 phase is a characteristic feature of mouse embryonic stem cells (ESCs). To determine if there is a causal relationship between G1 phase restriction and pluripotency, we made use of the Fluorescence Ubiquitination Cell Cycle Indicator (FUCCI) reporter system to FACS-sort ESCs in the different cell cycle phases. Hence, the G1 phase cells appeared to be more susceptible to differentiation, particularly when ESCs self-renewed in the naive state of pluripotency. Transitions from ground to naive, then from naive to primed states of pluripotency were associated with increased durations of the G1 phase, and cyclin E-mediated alteration of the G1 IS transition altered the balance between self-renewal and differentiation. LIE withdrawal resulted in a lengthening of the G1 phase in naive ESCs, which occurred prior to the appearance of early lineage-specific markers, and could be reversed upon LIE supplementation. We concluded that the short G1 phase observed in murine ESCs was a determinant of naive pluripotency and was partially under the control of LIE signaling. (C) 2012 Elsevier B.V. All rights reserved.