Fibroblasts are in a position to provide directional information to migrating neutrophils during pneumonia in rabbit lungs
Fibroblasts are in a position to provide directional information to migrating neutrophils during pneumonia in rabbit lungs
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DOI：
10.1006/mvre.1996.0029
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发表时间：
1996-05-01
影响因子：
3.1
通讯作者：
Walker, DC
中科院分区：
文献类型：
--
作者：
Behzad, AR;Chu, F;Walker, DC
先前的研究结果表明，肺成纤维细胞与内皮和上皮基底层中预先存在的孔有关，中性粒细胞在从毛细血管迁移到肺泡时似乎通过这些孔进入和离开间质。为了确定它们在中性粒细胞迁移中的作用，通过连续切片的兔肺组织的透射电子显微镜观察来评估间质内的成纤维细胞组织。发现间质成纤维细胞将内皮基底层孔与上皮基底层孔物理互连。对灌输肺炎链球菌的兔肺组织进行形态测量评估显示，迁移中性粒细胞表面积密度的约70%与间质成纤维细胞和细胞外基质成分紧密接触（15 nm或更小）（分别为30%和40%）。尽管迁移的中性粒细胞足够接近以粘附到成纤维细胞和细胞外成分，但间质成纤维细胞的组织方式允许它们向中性粒细胞提供方向信息。提出了一个说明该过程的模型。 (C) 1996 学术出版社
Previous findings have shown that pulmonary fibroblasts are associated with preexisting holes in the endothelial and epithelial basal laminae through which neutrophils appear to enter and leave the interstitium as they migrate from capillaries to alveoli. To determine their role in neutrophil migration, fibroblast organization within the interstitium was assessed by transmission electron microscope observations of serial-sectioned rabbit lung tissue. Interstitial fibroblasts were found to physically interconnect the endothelial basal lamina holes to epithelial basal lamina holes. Morphometric assessment of rabbit lung tissue instilled with Streptococcus pneumoniae revealed that approximately 70% of the surface area density of migrating neutrophils!s is in close contact (15 nm or less) with interstitial fibroblasts and extracellular matrix elements (30 and 40% respectively). Although migrating neutrophils were close enough to adhere to both fibroblasts and extracellular elements, the interstitial fibroblasts are organized in a manner that would allow them to provide directional information to the neutrophils. A model illustrating this process is proposed. (C) 1996 Academic Press, Inc.